Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4144-54. doi: 10.1016/j.msec.2013.06.004. Epub 2013 Jun 12.
The aim of this work was to investigate the effect of various sterilization methods on surface characteristics and biocompatibility of MgCa alloy, with pure Mg as a comparison, including steam autoclave sterilization (SA), ethylene oxide steam sterilization (EO), glutaraldehyde sterilization (GD), dry heat sterilization (DH) and Co60 γ ray radiation sterilization (R) technologies. The surface characterizations were performed by environmental scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, grazing incidence X-ray diffraction, water contact angle and surface free energy measurement, whereas the cytotoxicity and hemocompatibility were evaluated by cellular adhesive experiment, platelet adhesion and hemolysis test. The results showed that the five sterilization processes caused more changes on the surface of MgCa alloy than that on the surface of pure Mg. The GD sterilization caused the most obvious changes on the surface of the pure Mg, and the SA sterilization made the largest alteration on the MgCa alloy surface. The GD and DH sterilization processes could cause increases on surface free energy for both pure Mg and MgCa alloys, while the other three sterilization processes reduced the surface free energy. The DH and GD sterilization processes caused the least alteration on the cell adhesion on pure Mg surface, whereas the EO sterilization performed the greatest impact on the cell adhesion on the Mg-Ca alloy surface. The hemolysis percentage of pure Mg and MgCa alloys were reduced by SA sterilization, meanwhile the other four sterilization processes increased their hemolysis percentages significantly, especially for the EO sterilization.
本工作旨在研究不同灭菌方法对 MgCa 合金表面特性和生物相容性的影响，以纯 Mg 作为对照，包括蒸汽高压灭菌（SA）、环氧乙烷蒸汽灭菌（EO）、戊二醛灭菌（GD）、干热灭菌（DH）和 Co60γ射线辐射灭菌（R）技术。采用环境扫描电子显微镜、能谱仪、X 射线光电子能谱、掠入射 X 射线衍射、水接触角和表面自由能测量对表面特性进行了表征，而细胞黏附实验、血小板黏附和溶血试验则评估了细胞毒性和血液相容性。结果表明，五种灭菌工艺对 MgCa 合金表面的影响比对纯 Mg 表面的影响更大。GD 灭菌对纯 Mg 表面的影响最明显，SA 灭菌对 MgCa 合金表面的改变最大。GD 和 DH 灭菌过程会导致纯 Mg 和 MgCa 合金的表面自由能增加，而其他三种灭菌过程会降低表面自由能。DH 和 GD 灭菌过程对纯 Mg 表面细胞黏附的影响最小，而 EO 灭菌对 Mg-Ca 合金表面细胞黏附的影响最大。SA 灭菌会降低纯 Mg 和 MgCa 合金的溶血率，而其他四种灭菌过程则显著增加了它们的溶血率，尤其是 EO 灭菌。
