Chromy V, Kulhánek V, Fischer J
Clin Chem. 1978 Feb;24(2):379-81.
A new amine buffer for the pH range 8.5 to 10.5 based on D(-)-N-methylglucamine and its hydrochloride is described. Important physical and chemical constants characterizing this buffer system have been determined: thermodynamic pKa = 9.52 (25 degrees C), buffer capacity = 0.055, dilution value pH 1/2 = -0.02, temperature coefficient dpKa/dT = 0.023, the heat of ionization delta H degrees = 36.93 kj mol-1. D(-)-N-Methylglucamine, which is available in highly purified form, is highly soluble in water. Thus one can prepare either buffer solutions or stable solid mixtures when combined with D(-)-N-methylglucaminium chloride. D(-)-N-Methylglucamine buffers are suitable for both biochemistry and pH control.
描述了一种基于D(-)-N-甲基葡糖胺及其盐酸盐的新型pH范围为8.5至10.5的胺缓冲剂。已确定了表征该缓冲系统的重要物理和化学常数：热力学pKa = 9.52（25℃），缓冲容量 = 0.055，稀释值pH 1/2 = -0.02，温度系数dpKa/dT = 0.023，电离热ΔH° = 36.93 kJ mol-1。D(-)-N-甲基葡糖胺以高纯度形式可得，在水中高度可溶。因此，与D(-)-N-甲基葡糖胺氯化物结合时，可以制备缓冲溶液或稳定的固体混合物。D(-)-N-甲基葡糖胺缓冲剂适用于生物化学和pH控制。
