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利用免疫磁分离和直接荧光抗体染色法同时检测土壤中的蓝氏贾第鞭毛虫和微小隐孢子虫卵囊。

Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining.

机构信息

Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990 Israel.

出版信息

J Microbiol Methods. 2013 Sep;94(3):375-7. doi: 10.1016/j.mimet.2013.07.019. Epub 2013 Jul 31.

DOI:10.1016/j.mimet.2013.07.019
PMID:23911570
Abstract

An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g(-1) for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g(-1) soil.

摘要

描述了一种改进的方法，用于同时检测土壤中的微小隐孢子虫和蓝氏贾第鞭毛虫（卵囊）。对于浓度分别为>55 和 21（卵囊）/克的微小隐孢子虫和蓝氏贾第鞭毛虫，回收率>70%。检测限确定为<5（卵囊）/克土壤。

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