Virologie et Immunologie Moléculaires - INRA, Jouy en Josas, France.
Biochem Biophys Res Commun. 2013 Aug 30;438(3):519-25. doi: 10.1016/j.bbrc.2013.07.104. Epub 2013 Aug 1.
Lipid membrane can enhance prion protein (PrP) pathological fibrillogenesis. A neuronal paralog of PrP, named Shadoo (Sho), is localized to similar membrane environment as PrP and can also convert to amyloid-like fibrilles. To gain insight into the role of Sho in prion diseases, we studied Sho interactions with cellular membrane models. Sho was found to bind anionic lipid vesicles. Spectroscopic and microscopic data showed that membrane-associated Sho slowly converted into amyloid fibers. Furthermore, binding of Sho to anionic liposomes has a disruptive effect on the integrity of the lipid bilayer leading to the formation of supramolecular lipid-protein complexes. In consequence, the role of Sho in prion diseases might depend on the oligomerization state of Sho but also the nature of these lipoprotein assembles.
脂膜可以增强朊病毒蛋白（PrP）病理性纤维形成。PrP 的一种神经元同源物，名为 Shadoo（Sho），定位于类似于 PrP 的膜环境中，也可以转化为类似淀粉样的纤维。为了深入了解 Sho 在朊病毒疾病中的作用，我们研究了 Sho 与细胞膜模型的相互作用。发现 Sho 与阴离子脂质体结合。光谱和显微镜数据表明，膜结合的 Sho 缓慢转化为淀粉样纤维。此外，Sho 与阴离子脂质体的结合对脂质双层的完整性具有破坏作用，导致形成超分子脂质-蛋白质复合物。因此，Sho 在朊病毒疾病中的作用可能取决于 Sho 的寡聚状态，但也取决于这些脂蛋白组装的性质。
