Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 J2-15 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Appl Microbiol Biotechnol. 2014 Feb;98(4):1853-61. doi: 10.1007/s00253-013-5107-3. Epub 2013 Aug 3.
Oil souring has important implications with respect to energy resources. Understanding the physiology of the microorganisms that play a role and the biological mechanisms are both important for the maintenance of infrastructure and mitigation of corrosion processes. The objective of this study was to identify crude-oil components and microorganisms in oil-field water that contribute to crude-oil souring. To identify the crude-oil components and microorganisms that are responsible for anaerobic souring in oil reservoirs, biological conversion of crude-oil components under anaerobic conditions was investigated. Microorganisms in oil field water in Akita, Japan degraded alkanes and aromatics to volatile fatty acids (VFAs) under anaerobic conditions, and fermenting bacteria such as Fusibacter sp. were involved in VFA production. Aromatics such as toluene and ethylbenzene were degraded by sulfate-reducing bacteria (Desulfotignum sp.) via the fumarate-addition pathway and not only degradation of VFA but also degradation of aromatics by sulfate-reducing bacteria was the cause of souring. Naphthenic acid and 2,4-xylenol were not converted.
油的酸化对能源资源具有重要意义。了解起作用的微生物的生理学和生物机制对于基础设施的维护和腐蚀过程的缓解都是很重要的。本研究的目的是确定油田水中导致原油酸化的原油成分和微生物。为了确定导致油藏中厌氧酸化的原油成分和微生物，研究了在厌氧条件下原油成分的生物转化。日本秋田油田水中的微生物在厌氧条件下将烷烃和芳烃转化为挥发性脂肪酸（VFAs），发酵细菌如 Fusibacter sp. 参与了 VFA 的生成。芳烃如甲苯和乙苯通过延胡索酸添加途径被硫酸盐还原菌（Desulfotignum sp.）降解，不仅是 VFA 的降解，硫酸盐还原菌对芳烃的降解也是酸化的原因。环烷酸和 2,4-二甲酚没有被转化。
