Youngblood Victoria, Taylor James G
Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2013;1027:139-55. doi: 10.1007/978-1-60327-369-5_6.
The discovery of novel genetic variants and mutations in lipoprotein and cardiovascular disease research requires DNA sequencing. Large-scale genomics facilities will increasingly accomplish this with a combination of "next-generation" DNA sequencing methodologies. However, laboratories with limited access to these emerging technologies can still support focused genomic studies with the use of automated Sanger sequencing. Here, we describe two robust methods for medium-throughput DNA sequencing from PCR-amplified fragments of genomic DNA.
脂蛋白与心血管疾病研究中新型基因变异和突变的发现需要进行DNA测序。大规模基因组学设施将越来越多地通过“下一代”DNA测序方法的组合来完成这项工作。然而，难以使用这些新兴技术的实验室仍可通过自动化桑格测序来支持重点基因组研究。在此，我们描述了两种从基因组DNA的PCR扩增片段进行中通量DNA测序的可靠方法。
