Vaisman Boris L, Remaley Alan T
Lipoprotein Metabolism Section, Cardiovascular-Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2013;1027:343-52. doi: 10.1007/978-1-60327-369-5_16.
Lecithin-cholesterol acyltransferase (LCAT) is the major enzyme responsible for the esterification of free cholesterol on plasma lipoproteins, which is a key step in the reverse cholesterol transport pathway. The measurement of plasma LCAT activity not only is important in the diagnosis of patients with genetic or acquired LCAT deficiency but is also valuable in calculating cardiovascular risk, as well as in research studies of lipoprotein metabolism. In this chapter, we describe a convenient LCAT assay based on the use of an apoA-I mimetic peptide. The proteoliposome substrate used in this assay for LCAT is easily made with the peptide and can be stored by deep freezing without significant loss of activity.
卵磷脂胆固醇酰基转移酶（LCAT）是负责血浆脂蛋白上游离胆固醇酯化的主要酶，这是逆向胆固醇转运途径中的关键步骤。血浆LCAT活性的测定不仅对遗传性或获得性LCAT缺乏症患者的诊断很重要，而且在计算心血管风险以及脂蛋白代谢的研究中也很有价值。在本章中，我们描述了一种基于使用载脂蛋白A-I模拟肽的便捷LCAT测定方法。该测定中用于LCAT的蛋白脂质体底物很容易用该肽制成，并且可以通过深度冷冻保存而不会有明显的活性损失。
