Orozco-Nunnelly Danielle A, Kaufman Lon S, Warpeha Katherine M
Molecular, Cell, and Developmental Biology, University of Illinois at Chicago, Chicago, IL, USA.
Methods Mol Biol. 2013;1043:89-101. doi: 10.1007/978-1-62703-532-3_10.
A seed is competent to respond to light soon after imbibition. A new developmental program begins in or on the ground where the young seedling may be exposed to heat, cold, drought, flooding (anoxia), salts, varying levels of visible light, and the topic of this paper, ultraviolet radiation. Herein what is described is a method for growing and maintaining seedlings, then methods of UV irradiation in order to measure discrete effects of UV wavelengths in signal transduction, very early in seedling development. The physiological response to an abiotic signal is partly dependent on the developmental state of the plant. Dark-grown seedlings of plant species possess young leaves or leaf primordia in a "suspended" state of development whereby exposure to sunlight, visible and UV, is required to initiate the leaf developmental program, including development of etioplasts or proplastids into fully functioning chloroplasts. In order for us to understand the initial and persisting effects of UV in seedlings, we "delay" light-induced development by carrying out all experiments in complete darkness between days 0 (seed) and day 7 (Arabidopsis). In this case, the UV regulation of a simple signaling pathway in Arabidopsis, G protein signaling in UV protection and acclimation early in development, is investigated with the use of several mutants and easily score-able phenotypes.
种子在吸胀后不久就有能力对光作出反应。一个新的发育程序在地面上或地面开始，在那里幼苗可能会暴露于热、冷、干旱、洪水（缺氧）、盐、不同水平的可见光以及本文的主题——紫外线辐射。本文描述了一种培育和养护幼苗的方法，以及紫外线照射方法，以便在幼苗发育的早期阶段测量紫外线波长在信号转导中的离散效应。对非生物信号的生理反应部分取决于植物的发育状态。植物物种的暗生长幼苗具有处于“暂停”发育状态的幼叶或叶原基，因此需要暴露于阳光（可见光和紫外线）下才能启动叶片发育程序，包括将黄化质体或前质体发育成功能完全正常的叶绿体。为了理解紫外线对幼苗的初始和持续影响，我们在第0天（种子）至第7天（拟南芥）期间在完全黑暗的环境中进行所有实验，从而“延迟”光诱导的发育。在这种情况下，利用几种突变体和易于评分的表型，研究了拟南芥中一个简单信号通路的紫外线调节，即发育早期紫外线保护和适应中的G蛋白信号传导。
