Greenaway James B, Petrik Jim J
Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada.
Methods Mol Biol. 2013;1049:409-23. doi: 10.1007/978-1-62703-547-7_31.
One of the difficulties in studying ovarian cancer historically has been the lack of a suitable animal model that replicates the human disease. Mouse models that utilize intraperitoneal implantation of tumorigenic cells lack interaction between the transformed ovarian epithelial cells and the ovarian stroma, which we have shown to be an integral component in replicating the etiology seen in human epithelial ovarian cancer (Greenaway, Gynecol Oncol 108:385-394, 2008). Xenograft models generally require the use of immunocompromised hosts, which then eliminates the influence of the immune system in disease progression, which also has been shown to be an important part of the progression of epithelial ovarian cancer (EOC). In this chapter, we describe the generation and optimization of an orthotopic, syngeneic mouse model and illustrate the importance of facilitating epithelial-stromal cell interaction to more closely replicate human EOC.
从历史上看，研究卵巢癌的困难之一在于缺乏一种能复制人类疾病的合适动物模型。利用肿瘤细胞腹腔内植入的小鼠模型缺乏转化的卵巢上皮细胞与卵巢基质之间的相互作用，而我们已经证明这种相互作用是复制人类上皮性卵巢癌病因的一个重要组成部分（Greenaway，《妇科肿瘤学》108:385 - 394，2008年）。异种移植模型通常需要使用免疫缺陷宿主，这就消除了免疫系统在疾病进展中的影响，而免疫系统也已被证明是上皮性卵巢癌（EOC）进展的重要部分。在本章中，我们描述了一种原位同基因小鼠模型的建立和优化，并阐述了促进上皮 - 基质细胞相互作用以更紧密地复制人类EOC的重要性。
