Nunez-Cruz Selene, Scholler Nathalie
Department of Obstetrics and Gynecology, Penn Ovarian Cancer Research Center, University of Pennsylvania, Philadelphia, PA, USA.
Methods Mol Biol. 2013;1049:425-33. doi: 10.1007/978-1-62703-547-7_32.
Syngeneic and transgenic mouse models are important tools for the study of the biology of cancer. While syngeneic mouse models are generated through the implantation in host animals of tumor cells from genetically and immunologically compatible donors, transgenic mouse models are engineered to express genetic material with oncogenic properties in predetermined location. We have developed a syngeneic mouse model of ovarian cancer permitting in vivo imaging in immunocompetent recipients by implanting ovaries with fluorescently labeled cancer cells that derived from a spontaneous ovarian tumor developing in a transgenic mouse model. Tumor cells were retrovirally transduced with a far-red fluorescent protein. This animal model combines the advantages of syngeneic and transgenic mouse models as it permits to both monitor tumor growth by in vivo imaging and to analyze the tumor microenvironment of an immunocompetent host.
同基因和转基因小鼠模型是研究癌症生物学的重要工具。同基因小鼠模型是通过将来自基因和免疫兼容供体的肿瘤细胞植入宿主动物而产生的，而转基因小鼠模型则经过工程改造，使其在预定位置表达具有致癌特性的遗传物质。我们通过将携带源自转基因小鼠模型中自发发生的卵巢肿瘤的荧光标记癌细胞的卵巢植入免疫活性受体中，开发了一种卵巢癌同基因小鼠模型，该模型允许在免疫活性受体中进行体内成像。肿瘤细胞用远红色荧光蛋白进行逆转录病毒转导。这种动物模型结合了同基因和转基因小鼠模型的优点，因为它既可以通过体内成像监测肿瘤生长，又可以分析免疫活性宿主的肿瘤微环境。
