Huo J, Yang G, Zhang Y, Li F
College of Horticulture, Northeast Agricultural University, Harbin, China.
Genet Mol Res. 2013 Jun 21;12(2):2056-67. doi: 10.4238/2013.June.21.1.
We developed a new approach using RAPD fingerprints to distinguish 37 currant cultivars from northeastern China based on optimization of RAPD by choosing 11 nucleotide primers and strict screening PCR annealing temperature. We found that the manual cultivar identification diagram (MCID) approach clearly developed fingerprints from 8 different primers that were useful for cultivar identification; a cultivar identification diagram (CID) was readily constructed. This CID allows efficient currant cultivar identification, providing information to separate all the currant cultivars from each other, based on the detail polymorphic bands from the corresponding primers, which were marked in the correct positions on the currant CID. According to the CID, 10 currant cultivars in 5 groups were randomly selected for the referable and workable identification of this strategy. The results proved the workability and efficiency of the MCID method, facilitating the identification of fruit cultivars with DNA markers. This MCID approach will be useful for early identification of seedlings in the nursery industry and protection of cultivar rights.
我们开发了一种新方法，通过随机扩增多态性DNA（RAPD）指纹图谱来区分来自中国东北地区的37个醋栗品种。该方法基于对RAPD的优化，选择了11种核苷酸引物并严格筛选PCR退火温度。我们发现，手工品种鉴定图（MCID）方法通过8种不同引物清晰地生成了指纹图谱，这些指纹图谱可用于品种鉴定；据此构建了品种鉴定图（CID）。该CID能够高效地鉴定醋栗品种，基于相应引物产生的详细多态性条带，这些条带在醋栗CID上标记于正确位置，从而提供信息将所有醋栗品种彼此区分开来。根据该CID，从5组中随机选择了10个醋栗品种，以验证该策略的可参考性和可行性。结果证明了MCID方法的可行性和有效性，有助于利用DNA标记鉴定水果品种。这种MCID方法将有助于苗圃行业幼苗的早期鉴定和品种权的保护。
