Li Y H, Li M, He W Q, Wang Y G, Shao R G
School of Life Sciences and Technology, Henan Cultivating Key Laboratory of Hereditary Disease Targeted Molecular Drugs, Xinxiang Medical University, Xinxiang, China.
Genet Mol Res. 2013 Jun 21;12(2):2076-85. doi: 10.4238/2013.June.21.3.
The putative polyketide biosynthesis (PKS) genes cos10 and pg10 were inactivated by insertion of a kanamycin-resistance gene into the genome of the geldanamycin-producing strain, Streptomyces hygroscopicus 17997. The resultant inactivation were confirmed by PCR analysis. The abilities of the PKS gene inactivation strains to produce geldanamycin were compared with the natural geldanamycin- producing strain, S. hygroscopicus 17997. The cos10-inactivated strain exhibited an unchanged ability to produce geldanamycin, but the pg10- inactivated strain can produce twice the yield of the natural strain when grown under the same conditions. We propose that there is a sub-PKS pathway in the geldanamycin-producing strain, S. hygroscopicus 17997.
通过将卡那霉素抗性基因插入到格尔德霉素产生菌吸水链霉菌17997的基因组中，使假定的聚酮生物合成（PKS）基因cos10和pg10失活。通过PCR分析证实了由此产生的失活。将PKS基因失活菌株产生格尔德霉素的能力与天然产生格尔德霉素的菌株吸水链霉菌17997进行了比较。cos10失活菌株产生格尔德霉素的能力未发生变化，但pg10失活菌株在相同条件下生长时，其产量是天然菌株的两倍。我们推测在吸水链霉菌17997这一格尔德霉素产生菌株中存在一条亚聚酮合酶途径。
