新型4,5-二氢噻嗪诺加霉素在吸水链霉菌17997的gdmP突变株中的研究

Novel 4,5-dihydro-thiazinogeldanamycin in a gdmP mutant strain of Streptomyces hygroscopicus 17997.

作者信息

Lin Ling, Ni SiYang, Wu LinZhuan, Wang YiGuang, Wang YuCheng, Tao PeiZhen, He WeiQing, Wang XiangJing

机构信息

Northeast Agricultural University, Harbin, China.

出版信息

Biosci Biotechnol Biochem. 2011;75(10):2042-5. doi: 10.1271/bbb.110361. Epub 2011 Oct 7.

DOI:10.1271/bbb.110361

PMID:21979086

Abstract

Novel geldanamycin derivative, 4,5-dihydro-thiazinogeldanamycin (3), was characterized from the gdmP mutant in Streptomyces hygroscopicus 17997, besides expected 4,5-dihydro-geldanamycin (2). The presence of this compound would suggest an unknown post-PKS modification in geldanamycin biosynthesis. Compound 3 exhibited moderate anti-HSV-1-virus activity and higher water solubility than geldanamycin (1). Cysteine served as a precursor to synthesize 3, whose formation required obligatory enzymatic assistance.

摘要

新型格尔德霉素衍生物4,5-二氢噻嗪格尔德霉素（3），是从吸水链霉菌17997的gdmP突变体中鉴定出来的，此外还鉴定出了预期的4,5-二氢格尔德霉素（2）。该化合物的存在表明格尔德霉素生物合成中存在未知的聚酮合酶后修饰。化合物3表现出中等的抗单纯疱疹病毒1型（HSV-1）活性，且水溶性高于格尔德霉素（1）。半胱氨酸作为合成3的前体，其形成需要特定的酶促作用。