Terabayashi T, Ogawa T, Kawanishi Y
Department of Chemistry, Kitasato University, Kanagawa.
J Biochem. 1990 Jun;107(6):868-71. doi: 10.1093/oxfordjournals.jbchem.a123140.
Two novel ganglioside lactones were isolated from the brain of Bryde's whale (Balaenoptera edeni) and characterized. These gangliosides migrated above GM4 and were designated M4-1 and M4-2 in the order of location from the bottom of the TLC plate. These gangliosides were converted to GM4 by mild alkali treatment. Although M4-1 and M4-2 contained 1 mol each of N-acetylneuraminic acid and galactose, they behaved as neutral lipids on ion-exchange chromatography. Inner ester linkages were found in these gangliosides by secondary ion mass spectrometry and infrared spectroscopy. Two-dimensional J-correlated proton NMR spectra revealed that an inner ester linkage was formed in M4-1 between the sialic acid carboxyl group and the C-4 hydroxyl group of the galactosyl residue and another inner ester linkage was formed in M4-2 between the sialic acid carboxyl group and the C-2 hydroxyl group of galactose.
从布氏鲸（Balaenoptera edeni）的大脑中分离并鉴定出两种新型神经节苷脂内酯。这些神经节苷脂在GM4上方迁移，并根据其在薄层层析板底部的位置顺序分别命名为M4-1和M4-2。通过温和的碱处理，这些神经节苷脂可转化为GM4。尽管M4-1和M4-2各含有1摩尔的N-乙酰神经氨酸和半乳糖，但它们在离子交换色谱中表现为中性脂质。通过二次离子质谱和红外光谱在这些神经节苷脂中发现了内部酯键。二维J-相关质子核磁共振谱显示，M4-1中唾液酸羧基与半乳糖基残基的C-4羟基之间形成了一个内部酯键，而M4-2中唾液酸羧基与半乳糖的C-2羟基之间形成了另一个内部酯键。
