利用电离辐射使核蛋白与DNA交联。
The crosslinking of nuclear protein to DNA using ionizing radiation.
作者信息
Cress A E, Kurath K M, Stea B, Bowden G T
机构信息
Department of Radiation Oncology, University of Arizona Cancer Center, Tucson 85724.
出版信息
J Cancer Res Clin Oncol. 1990;116(4):324-30. doi: 10.1007/BF01612913.
DNA-protein complexes were generated in intact Chinese hamster ovary (CHO) cells by the use of ionizing radiation. The DNA-protein crosslinks, as measured by a filter-binding assay, occurred immediately following the irradiation, were produced in a dose-dependent manner and were reversible. The reversibility of the crosslinks in the intact cells was dependent upon general protein synthesis. Three proteins that were attached to DNA in unirradiated cells were analyzed according to the presence of DNA attached to the proteins before, during and after exposure to ionizing radiation. All three proteins contained more DNA reversibly attached to the proteins after exposure to 5 Gy ionizing radiation as compared to unirradiated cells. One of the proteins was increasingly attached to DNA using 2.5-50 Gy X-ray. These data suggest that the increased DNA-protein crosslinking observed with ionizing radiation may involve the increase in particular protein(s) crosslinked to DNA as well as an increase in the amount of DNA attached to specific proteins.
通过使用电离辐射在完整的中国仓鼠卵巢（CHO）细胞中生成DNA - 蛋白质复合物。通过滤膜结合测定法测量，DNA - 蛋白质交联在照射后立即发生，呈剂量依赖性产生且是可逆的。完整细胞中交联的可逆性取决于一般蛋白质合成。根据在暴露于电离辐射之前、期间和之后与蛋白质相连的DNA的存在情况，对未照射细胞中与DNA相连的三种蛋白质进行了分析。与未照射细胞相比，所有三种蛋白质在暴露于5 Gy电离辐射后都含有更多可逆地与蛋白质相连的DNA。其中一种蛋白质使用2.5 - 50 Gy X射线时与DNA的连接不断增加。这些数据表明，电离辐射观察到的DNA - 蛋白质交联增加可能涉及与DNA交联的特定蛋白质的增加以及与特定蛋白质相连的DNA量的增加。
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