利用转基因小鼠中的青色荧光报告基因分析原始红细胞的增殖和去核过程。
Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice.
作者信息
Vacaru Andrei M, Isern Joan, Fraser Stuart T, Baron Margaret H
机构信息
Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, New York; The Tisch Cancer Institute, The Icahn School of Medicine at Mount Sinai, New York, New York.
出版信息
Genesis. 2013 Nov;51(11):751-62. doi: 10.1002/dvg.22420. Epub 2013 Aug 30.
Abstract
Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage.
摘要
原始红细胞生成是哺乳动物胚胎发育的一个重要过程。在此，我们报告一种新的转基因小鼠品系的产生及特性，该品系在原始红细胞的细胞核中表达组蛋白H2B-CFP融合蛋白。我们展示了这种ε-珠蛋白-组蛋白H2B-CFP品系在多色成像和流式细胞术分析方面的潜力。利用ε-珠蛋白-H2B-CFP品系分析了胚胎第8.5天至13.5天表达CFP的原始成红细胞的细胞周期分布和增殖情况。我们还评估了胎儿肝脏和胎盘中巨噬细胞对挤出的CFP阳性细胞核的吞噬作用。ε-珠蛋白-H2B-CFP转基因小鼠品系为研究原始红细胞谱系的发育增添了可用工具。
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