Vacaru Andrei M, Isern Joan, Fraser Stuart T, Baron Margaret H
Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, New York; The Tisch Cancer Institute, The Icahn School of Medicine at Mount Sinai, New York, New York.
Genesis. 2013 Nov;51(11):751-62. doi: 10.1002/dvg.22420. Epub 2013 Aug 30.
Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage.
原始红细胞生成是哺乳动物胚胎发育的一个重要过程。在此，我们报告一种新的转基因小鼠品系的产生及特性，该品系在原始红细胞的细胞核中表达组蛋白H2B-CFP融合蛋白。我们展示了这种ε-珠蛋白-组蛋白H2B-CFP品系在多色成像和流式细胞术分析方面的潜力。利用ε-珠蛋白-H2B-CFP品系分析了胚胎第8.5天至13.5天表达CFP的原始成红细胞的细胞周期分布和增殖情况。我们还评估了胎儿肝脏和胎盘中巨噬细胞对挤出的CFP阳性细胞核的吞噬作用。ε-珠蛋白-H2B-CFP转基因小鼠品系为研究原始红细胞谱系的发育增添了可用工具。
