Yasuno Hironobu, Watanabe Takeshi, Miyamoto Yumiko, Kandori Hitoshi, Yamasaki Hideki, Fukuda Ryo
Drug Safety Research Laboratories, Takeda Pharmaceutical Company Limited, 26-1 Muraoka-Higashi 2-Chome, Fujisawa, Kanagawa 251-8555, Japan.
J Toxicol Pathol. 2013 Jun;26(2):209-13. doi: 10.1293/tox.26.209. Epub 2013 Jul 10.
The protein expressions of steroidogenic factor l (SF-l) and pituitary-specific transcription factor 1 (Pit-1) were investigated immunohistochemically for 53 spontaneous pituitary adenomas of the pars distalis from male Crl:CD(SD) rats. Luteinizing hormone (LH)-positive/prolactin (PRL)-negative and LH-negative/PRL-positive adenomas showed that the expression of SF-1 and Pit-1 was exclusively related to the immunoreactivity of LH and PRL, respectively. All double-positive adenomas (positive for both LH and PRL) were positive for Pit-1 and were supposed to be derived from PRL cells, although some of them also showed SF-1 immunoreactivity. In addition, all null cell adenomas (negative for all anterior pituitary hormones) were positive for SF-1 and negative for Pit-1, indicating that they originated from the gonadotroph cell lineage. This is the first report focusing on the application of transcription factors for the classification of rat pituitary adenomas.
采用免疫组织化学方法，对53只雄性Crl:CD(SD)大鼠远侧部自发性垂体腺瘤进行了类固醇生成因子1（SF-1）和垂体特异性转录因子1（Pit-1）蛋白表达的研究。促黄体生成素（LH）阳性/催乳素（PRL）阴性及LH阴性/PRL阳性腺瘤显示，SF-1和Pit-1的表达分别仅与LH和PRL的免疫反应性相关。所有双阳性腺瘤（LH和PRL均阳性）Pit-1呈阳性，推测其起源于PRL细胞，尽管其中一些也显示出SF-1免疫反应性。此外，所有无功能细胞腺瘤（所有垂体前叶激素均为阴性）SF-1呈阳性，Pit-1呈阴性，表明它们起源于促性腺激素细胞系。这是首篇聚焦转录因子在大鼠垂体腺瘤分类中应用的报告。
