Nabian S, Taheri M, Fard R Mazaheri Nezhad, Aramoon M
Department of Parasitology and Research Center of Ticks and Tick Borne Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Parasitol. 2013 Apr;8(2):242-8.
Boophilus annulatus is an obligate blood feeder tick that can cause great losses in animals due to anemia and its ability to injure its host skin directly. The aim of this study was identification of cattle humoral immune response to some tick proteins during experimental infestation.
Immune sera against tick were collected from experimentally infested cattle with ticks. One and two-dimensional electrophoresis and Western blotting methods were used for the detection of immunogenic proteins in larval tick extract and eight of these proteins were identified by MALDITOF and MALDI-TOF-TOF mass spectrometry.
In non-reducing one-dimensional SDS-PAGE, some bounds between 12 to more than 250-kDa appeared. In two-dimensional SDS-PAGE, numerous spot appeared and the identified immunogenic proteins by parallel immunoblotting weighted between 14 and 97 kDa. Amino acid sequences of protein spot with 37-kDa molecular weight had identity to tropomyosin based on Mascot search in NCBI.
Anti tropomyosin antibodies can be induced in experimentally infested hosts with ticks and it seems that tropomyosin can be useful for the development of anti tick vaccines.
环形牛蜱是一种专性吸血蜱，可因贫血及其直接损伤宿主皮肤的能力而给动物造成巨大损失。本研究的目的是鉴定实验性感染期间牛对某些蜱蛋白的体液免疫反应。
从实验性感染蜱的牛身上采集抗蜱免疫血清。采用一维和二维电泳以及蛋白质印迹法检测蜱幼虫提取物中的免疫原性蛋白，其中8种蛋白通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和基质辅助激光解吸电离串联飞行时间质谱（MALDI-TOF-TOF）进行鉴定。
在非还原一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）中，出现了一些12至超过250 kDa之间的条带。在二维SDS-PAGE中，出现了大量斑点，通过平行免疫印迹鉴定的免疫原性蛋白重量在14至97 kDa之间。基于NCBI中的Mascot搜索，分子量为37 kDa的蛋白斑点的氨基酸序列与原肌球蛋白具有同一性。
在实验性感染蜱的宿主中可诱导产生抗原肌球蛋白抗体，原肌球蛋白似乎可用于抗蜱疫苗的研发。
