Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.
Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
Infect Agent Cancer. 2013 Aug 2;8(1):29. doi: 10.1186/1750-9378-8-29.
Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro native EBV-infected NPC cell model commonly used for study of the viral-host interaction. Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model has not been characterized.
To determine the complete EBV genome sequence in C666-1 cells.
The C666-1 genome was sequenced by 100-bases pair-end massive parallel sequencing. Bioinformatics analysis was performed to extract the EBV sequences and construct an EBV consensus sequence map. PCR amplification and Sanger DNA sequencing were used for sequence validation and gap filling. A phylogenetic analysis of EBV strain in C666-1 cells and other reported EBV strains was performed.
A 171,317 bp complete EBV genome of C666-1 was successfully constructed (GenBank accession number: KC617875). Phylogenetic analysis of EBV genome in C666-1 revealed that the C666-1 EBV strain is closely related to the reported strains in NPC primary tumors.
C666-1 contains a representative NPC-associated EBV genome and might serve as an important model for studying the roles or function of viral proteins in NPC tumorigenesis.
鼻咽癌是一种独特的头颈部癌症，与 Epstein-Barr 病毒（EBV）密切相关。C666-1 细胞系是唯一常用的体外天然 EBV 感染 NPC 细胞模型，用于研究病毒-宿主相互作用。然而，该体外 EBV 感染 NPC 模型中的完整 EBV 基因组序列尚未得到描述。
确定 C666-1 细胞中的完整 EBV 基因组序列。
通过 100 碱基对末端大规模平行测序对 C666-1 基因组进行测序。生物信息学分析用于提取 EBV 序列并构建 EBV 共识序列图谱。PCR 扩增和 Sanger DNA 测序用于序列验证和缺口填充。对 C666-1 细胞中的 EBV 株与其他报道的 EBV 株进行系统发育分析。
成功构建了 171317bp 的 C666-1 完整 EBV 基因组（GenBank 登录号：KC617875）。C666-1 中 EBV 基因组的系统发育分析表明，C666-1 EBV 株与报道的 NPC 原发性肿瘤中的株密切相关。
C666-1 含有具有代表性的 NPC 相关 EBV 基因组，可能成为研究病毒蛋白在 NPC 肿瘤发生中的作用或功能的重要模型。
