新型组蛋白翻译后修饰的蛋白质组学特征。
Proteomic characterization of novel histone post-translational modifications.
机构信息
Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine University of Pennsylvania, 1009C Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104, USA.
出版信息
Epigenetics Chromatin. 2013 Aug 1;6(1):24. doi: 10.1186/1756-8935-6-24.
Abstract
Histone post-translational modifications (PTMs) have been linked to a variety of biological processes and disease states, thus making their characterization a critical field of study. In the last 5 years, a number of novel sites and types of modifications have been discovered, greatly expanding the histone code. Mass spectrometric methods are essential for finding and validating histone PTMs. Additionally, novel proteomic, genomic and chemical biology tools have been developed to probe PTM function. In this snapshot review, proteomic tools for PTM identification and characterization will be discussed and an overview of PTMs found in the last 5 years will be provided.
摘要
组蛋白翻译后修饰（PTMs）与多种生物过程和疾病状态有关，因此其特征描述是一个关键的研究领域。在过去的 5 年中，已经发现了许多新的位点和类型的修饰，大大扩展了组蛋白密码。质谱方法对于发现和验证组蛋白 PTM 至关重要。此外，还开发了新的蛋白质组学、基因组学和化学生物学工具来探测 PTM 功能。在这个快照综述中，将讨论用于 PTM 鉴定和表征的蛋白质组学工具，并提供过去 5 年中发现的 PTM 的概述。
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