Xiang Yuzhu, Qiu Qingchao, Jiang Ming, Jin Renjie, Lehmann Brian D, Strand Douglas W, Jovanovic Bojana, DeGraff David J, Zheng Yi, Yousif Dina A, Simmons Christine Q, Case Thomas C, Yi Jia, Cates Justin M, Virostko John, He Xiusheng, Jin Xunbo, Hayward Simon W, Matusik Robert J, George Alfred L, Yi Yajun
Department of Medicine, Vanderbilt University, Nashville, TN 37232-0275, USA; Minimally Invasive Urology Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Mol Oncol. 2013 Dec;7(6):1019-30. doi: 10.1016/j.molonc.2013.07.008. Epub 2013 Jul 20.
Metastasis, the main cause of death from cancer, remains poorly understood at the molecular level.
Based on a pattern of reduced expression in human prostate cancer tissues and tumor cell lines, a candidate suppressor gene (SPARCL1) was identified. We used in vitro approaches to determine whether overexpression of SPARCL1 affects cell growth, migration, and invasiveness. We then employed xenograft mouse models to analyze the impact of SPARCL1 on prostate cancer cell growth and metastasis in vivo.
SPARCL1 expression did not inhibit tumor cell proliferation in vitro. By contrast, SPARCL1 did suppress tumor cell migration and invasiveness in vitro and tumor metastatic growth in vivo, conferring improved survival in xenograft mouse models.
We present the first in vivo data suggesting that SPARCL1 suppresses metastasis of prostate cancer.
转移是癌症致死的主要原因，在分子水平上仍未得到充分理解。
基于人类前列腺癌组织和肿瘤细胞系中表达降低的模式，鉴定出一个候选抑癌基因（SPARCL1）。我们采用体外方法来确定SPARCL1的过表达是否影响细胞生长、迁移和侵袭性。然后我们利用异种移植小鼠模型来分析SPARCL1对体内前列腺癌细胞生长和转移的影响。
SPARCL1的表达在体外并不抑制肿瘤细胞增殖。相比之下，SPARCL1在体外确实抑制肿瘤细胞迁移和侵袭性，在体内抑制肿瘤转移生长，在异种移植小鼠模型中提高了生存率。
我们提供了首个体内数据，表明SPARCL1抑制前列腺癌转移。
