Department of Experimental Medicine and Surgery, "Tor Vergata" University Hospital, Rome, Italy.
J Neuroimmunol. 2013 Oct 15;263(1-2):116-20. doi: 10.1016/j.jneuroim.2013.07.006. Epub 2013 Aug 1.
Intrathecal immunoglobulin synthesis is observed in several disorders of the central nervous system, but its detection by current laboratory tests is relatively insensitive and operator depending. We assessed the diagnostic accuracy of a nephelometric assay for k free light chain determination in cerebrospinal fluid and serum. The patients were grouped according to clinical and laboratory criteria. ROC curves for all methods were performed to find the best cut-off value. kFLC Index seems to be more accurate than other parameters. Our data indicate that nephelometric assay for kFLCs in CSF reliably detect intrathecal immunoglobulin synthesis and discriminate multiple sclerosis patients.
鞘内免疫球蛋白合成可见于中枢神经系统的几种疾病中，但目前的实验室检测方法相对不敏感且依赖于操作者。我们评估了脑脊液和血清中游离轻链 k 测定的散射比浊法的诊断准确性。根据临床和实验室标准对患者进行分组。为了找到最佳截断值，对所有方法进行了 ROC 曲线分析。kFLC 指数似乎比其他参数更准确。我们的数据表明，CSF 中 kFLC 的散射比浊法可靠地检测到鞘内免疫球蛋白合成，并区分多发性硬化症患者。
