Department of Basic Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad 91779-48974, Iran.
Res Vet Sci. 2013 Dec;95(3):891-3. doi: 10.1016/j.rvsc.2013.07.010. Epub 2013 Aug 2.
Iodothyronine deiodinases I and II (DIO1 and DIO2) remove iodine from T4 to convert it to a more biologically active T3. The relative contribution of different tissue deiodinases to the establishment of a euthyroid state in sheep is not known. The objective of this study was to quantitate the amounts of transcription of DIO1 and DIO2 deiodinases in different ovine tissues. Using RT-qPCR, we found that DIO1 deiodinase is transcribed in skeletal muscle, kidney, and heart, more than thyroid, in diaphragm in quantities very similar to thyroid, and in liver, spleen, lung, and mammary gland lower than thyroid. We also found that the level of DIO2 transcription in all other tissues was lower than that in thyroid. In clinical settings, measurement of DIO1 and DIO2 expression in a given tissue may provide important clues on the intensity of selenium deficiency and its effects on the metabolism of thyroid hormones.
碘甲状腺原氨酸脱碘酶 I 和 II（DIO1 和 DIO2）从 T4 中去除碘，将其转化为更具生物活性的 T3。不同组织脱碘酶在绵羊建立甲状腺功能正常状态中的相对贡献尚不清楚。本研究的目的是定量检测不同绵羊组织中 DIO1 和 DIO2 脱碘酶的转录量。使用 RT-qPCR，我们发现 DIO1 脱碘酶在骨骼肌、肾脏和心脏中转录，在膈肌中的转录量与甲状腺相似，而在肝脏、脾脏、肺和乳腺中的转录量低于甲状腺。我们还发现，所有其他组织中 DIO2 的转录水平均低于甲状腺。在临床环境中，测量特定组织中 DIO1 和 DIO2 的表达可能为硒缺乏的强度及其对甲状腺激素代谢的影响提供重要线索。
