College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan Province, China.
Gene. 2013 Oct 10;528(2):59-66. doi: 10.1016/j.gene.2013.07.023. Epub 2013 Aug 1.
To explore the relevance of rat liver regeneration (LR) to acute hepatic failure (AHF), Rat Genome 230 2.0 Array was used to detect their gene expression profiles in this study, and the reliability of the detection results was confirmed by real-time-PCR. 1012 genes were found to be significantly changed in AHF occurrence and 948 genes in LR. Hierarchical clustering analysis showed that physiological activities of AHF and those of LR had no time correlation. Hierarchical clustering analysis (which is performed to group genes based on the similarity of expression patterns) showed that physiological activities of AHF and those of LR had no time correlation. K-means clustering analysis (which is used to check the difference in the relevant predictor variables between different groups is significant or not) demonstrated that gene expression trend of C1 group (genes relate to categories of stimulus-response and cell apoptosis, etc.) in AHF and in LR was extremely similar, that those of their C2 group (categories of regulation of homeostasis and hormone stimulation, etc.) were contrary, and that those of their C3 (material and energy metabolism and oxidation reduction, etc.), C4 (Cell cycle-related genes) and C5 (cell proliferation-related genes) groups were also similar with the gene expression changes of LR more abundant. GO classifications and functional clustering analysis (which was used to statistics the numbers or composition of proteins or genes at a function level) revealed that cellular processes including immune response, inflammatory reaction, cell migration and adhesion, etc. were increased both in AHF and in LR, whereas material and energy metabolism were decreased. Of them, stimulus response, inflammatory reaction and regulation of apoptosis, etc. were stronger in AHF occurrence than in LR, but ion homeostasis, hormonal response, regulation of cell division and proliferation, etc. were weaker in AHF occurrence. Gene expression changes and physiological activities of AHF and those of LR not only have similarities but also differences.
为了探索大鼠肝再生（LR）与急性肝衰竭（AHF）的相关性，本研究采用大鼠基因组 230 2.0 阵列检测其基因表达谱，并通过实时 PCR 验证检测结果的可靠性。在 AHF 发生时发现 1012 个基因发生显著变化，在 LR 中发现 948 个基因发生显著变化。层次聚类分析表明，AHF 和 LR 的生理活动没有时间相关性。层次聚类分析（用于根据表达模式的相似性对基因进行分组）表明，AHF 和 LR 的生理活动没有时间相关性。K-均值聚类分析（用于检查不同组之间相关预测变量的差异是否显著）表明，AHF 中 C1 组（与刺激反应和细胞凋亡等类别相关的基因）和 LR 中的基因表达趋势非常相似，其 C2 组（与内稳态调节和激素刺激等类别相关的基因）则相反，其 C3（物质和能量代谢及氧化还原等）、C4（细胞周期相关基因）和 C5（细胞增殖相关基因）组也与 LR 的基因表达变化相似，且 LR 的基因表达变化更为丰富。GO 分类和功能聚类分析（用于统计蛋白质或基因在功能水平上的数量或组成）表明，免疫反应、炎症反应、细胞迁移和粘附等细胞过程在 AHF 和 LR 中均增加，而物质和能量代谢减少。其中，刺激反应、炎症反应和凋亡调节等在 AHF 发生时比在 LR 中更为强烈，但离子内稳态、激素反应、细胞分裂和增殖调节等在 AHF 发生时较弱。AHF 和 LR 的基因表达变化和生理活动不仅有相似之处，也有差异。
