Food Toxicology Division, CSIR-Indian Institute of Toxicology Research (Formerly - Industrial Toxicology Research Centre), Mahatma Gandhi Marg, P.O. Box No. 80, Lucknow 226 001, India; Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi 110 062, India.
Cell Immunol. 2013 Jul-Aug;284(1-2):37-44. doi: 10.1016/j.cellimm.2013.07.001. Epub 2013 Jul 12.
Red kidney bean (Phaseolus vulgaris L.) is one the most commonly consumed legumes that requires an in depth understanding of its allergenicity. Therefore, the aim of this study was to explore the allergenicity of red kidney bean proteins following oral exposure in BALB/c mice and elucidate the levels of Th1/Th2 transcription factors induced by red kidney bean proteins in rat basophilic leukemia cells (RBL-2H3 cells) passively sensitized with the sera of red kidney bean sensitized mice. Red kidney bean proteins showed enhanced levels of total and specific IgE, anaphylactic symptoms, thymic stromal lymphopoietin (TSLP) and peritoneal albumin over control. Enhanced release of β-hexosaminidase along with up regulated expressions of GATA-3, STAT-6, T-bet, c-MAF and NFAT were observed in the RBL-2H3 cells exposed with red kidney bean proteins when compared to that of the controls. Taken together, exposure of red kidney bean proteins may cause allergic symptoms in mice and the ambivalent effect on Th2/Th1 transcription factors in RBL-2H3 cells.
红芸豆（Phaseolus vulgaris L.）是最常食用的豆类之一，对其致敏性需要深入了解。因此，本研究旨在探讨口服暴露于红芸豆蛋白后 BALB/c 小鼠的致敏性，并阐明用红芸豆致敏小鼠血清被动致敏的大鼠嗜碱性白血病细胞（RBL-2H3 细胞）中红芸豆蛋白诱导的 Th1/Th2 转录因子水平。与对照组相比，红芸豆蛋白表现出总 IgE 和特异性 IgE 水平升高、过敏症状、胸腺基质淋巴细胞生成素 (TSLP) 和腹腔白蛋白升高。在暴露于红芸豆蛋白的 RBL-2H3 细胞中，观察到β-己糖胺酶释放增强，同时 GATA-3、STAT-6、T-bet、c-MAF 和 NFAT 的表达上调。总之，红芸豆蛋白的暴露可能会导致小鼠出现过敏症状，并对 RBL-2H3 细胞中的 Th2/Th1 转录因子产生双重影响。
