Authors' Affiliations: Public Health Sciences Division, Clinical Research Division, Fred Hutchinson Cancer Research Center; Departments of Epidemiology, Pharmacy, and Pharmaceutics, University of Washington, Seattle, Washington; Division of Preventive Oncology, National Center for Tumor Diseases; Division of Epigenomics and Cancer Risk Factors, German Cancer Research Center, Heidelberg, Germany.
Cancer Epidemiol Biomarkers Prev. 2013 Dec;22(12):2312-22. doi: 10.1158/1055-9965.EPI-13-0470. Epub 2013 Aug 5.
Oxidative stress and resulting cellular damage have been suggested to play a role in the etiology of several chronic diseases, including cancer and cardiovascular disease. Identifying factors associated with reduced oxidative stress and resulting damage may guide future disease-prevention strategies.
In the VITamins And Lifestyle (VITAL) biomarker study of 209 persons living in the Seattle area, we examined the association between current use of several specialty supplements and oxidative stress, DNA damage, and DNA repair capacity. Use of glucosamine, chondroitin, fish oil, methylsulfonylmethane (MSM), coenzyme Q10 (CoQ10), ginseng, ginkgo, and saw palmetto was ascertained by a supplement inventory/interview, whereas the use of fiber supplements was ascertained by questionnaire. Supplements used by more than 30 persons (glucosamine and chondroitin) were evaluated as the trend across number of pills/week (non-use, <14 pills/week, 14+ pills/week), whereas less commonly used supplements were evaluated as use/non-use. Oxidative stress was measured by urinary 8-isoprostane and PGF2α concentrations using enzyme immunoassays (EIA), whereas lymphocyte DNA damage and DNA repair capacity were measured using the Comet assay. Multivariate-adjusted linear regression was used to model the associations between supplement use and oxidative stress/DNA damage.
Use of glucosamine (Ptrend: 0.01), chondroitin (Ptrend: 0.003), and fiber supplements (P: 0.01) was associated with reduced PGF2α concentrations, whereas CoQ10 supplementation was associated with reduced baseline DNA damage (P: 0.003).
Use of certain specialty supplements may be associated with reduced oxidative stress and DNA damage.
Further research is needed to evaluate the association between specialty supplement use and markers of oxidative stress and DNA damage.
氧化应激及其导致的细胞损伤被认为在多种慢性疾病（包括癌症和心血管疾病）的发病机制中起作用。确定与降低氧化应激及其导致的损伤相关的因素可能为未来的疾病预防策略提供指导。
在西雅图地区 209 名居民的维生素和生活方式（VITAL）生物标志物研究中，我们研究了当前使用几种特种补充剂与氧化应激、DNA 损伤和 DNA 修复能力之间的关联。通过补充剂清单/访谈确定了氨基葡萄糖、软骨素、鱼油、甲基磺酰甲烷（MSM）、辅酶 Q10（CoQ10）、人参、银杏和锯棕榈的使用情况，而通过问卷确定了纤维补充剂的使用情况。评估了使用人数超过 30 人的补充剂（氨基葡萄糖和软骨素）的趋势（非使用者、<14 片/周、14 片/周以上），而使用较少的补充剂则评估为使用者/非使用者。采用酶联免疫吸附试验（EIA）检测尿 8-异前列腺素和 PGF2α 浓度来衡量氧化应激，采用彗星试验检测淋巴细胞 DNA 损伤和 DNA 修复能力。采用多变量调整线性回归模型来分析补充剂使用与氧化应激/DNA 损伤之间的关联。
使用氨基葡萄糖（Ptrend：0.01）、软骨素（Ptrend：0.003）和纤维补充剂（P：0.01）与 PGF2α 浓度降低相关，而 CoQ10 补充剂与基线 DNA 损伤降低相关（P：0.003）。
某些特种补充剂的使用可能与降低氧化应激和 DNA 损伤相关。
需要进一步研究以评估特种补充剂使用与氧化应激和 DNA 损伤标志物之间的关联。
