Fujita S, Tonohata A
Central Clinical Laboratory, Kanazawa University Hospital.
Jpn J Antibiot. 1990 May;43(5):757-60.
The influence of Mueller-Hinton (MH) broth (from BBL Microbiology Systems, and Difco Laboratories) of minimum inhibitory concentrations (MIC) of cefuzoname (CZON), flomoxef (FMOX), imipenem (IPM), and minocycline (MINO) for 100 strains of Staphylococcus aureus was investigated. Antibacterial activity of MINO was stronger than any other antibiotics. MICs of CZON for 16 strains (14 of 50 methicillin-resistant S. aureus (MRSA), 2 of 50 methicillin-sensitive S. aureus) were greater than or equal to 4-fold greater when tested in BBL MH broth than when tested in Difco MH broth, thus, different media altered categories of some strains (8 of 50 MRSA) from susceptible to resistant. MICs of FMOX in the BBL MH broth for 12 of 50 MRSA strains rose greater than or equal to 4-fold compared to the Difco MH broth. On the other hand, MICs of IPM and MINO were affected very little by the different brand of MH broth used.
研究了Mueller-Hinton（MH）肉汤（购自BBL微生物系统公司和Difco实验室）对100株金黄色葡萄球菌的头孢唑南（CZON）、氟氧头孢（FMOX）、亚胺培南（IPM）和米诺环素（MINO）最低抑菌浓度（MIC）的影响。米诺环素的抗菌活性强于其他任何抗生素。当在BBL MH肉汤中测试时，16株菌（50株耐甲氧西林金黄色葡萄球菌（MRSA）中的14株，50株甲氧西林敏感金黄色葡萄球菌中的2株）的头孢唑南MIC比在Difco MH肉汤中测试时高出或等于4倍以上，因此，不同培养基改变了部分菌株（50株MRSA中的8株）的敏感类别，使其变为耐药。与Difco MH肉汤相比，50株MRSA菌株中的12株在BBL MH肉汤中的氟氧头孢MIC升高了4倍或以上。另一方面，所用MH肉汤品牌的不同对亚胺培南和米诺环素的MIC影响很小。
