Kojima Y
Laboratory of Reproductive Physiology, Faculty of Agriculture, Shizuoka University, Japan.
Nihon Juigaku Zasshi. 1990 Aug;52(4):781-6. doi: 10.1292/jvms1939.52.781.
The fine structure of tubulobulbar complex (TBC) of goat seminiferous epithelium was studied using testicular samples fixed by perfusion. During the maturation phase of the spermatid, the TBC appeared on the periphery of the head before spermiation, dorsal and ventral parts of the head, both sides of the acrosome and on the postnuclear sheath region. At an early stage of the development of the TBC, a partial loss of smooth endoplasmic reticulum in the ectoplasmic specialization of a neighboring Sertoli cell was observed; filament bundles disappeared and a small fragmented smooth endoplasmic reticulum was left. Subsequently, a spermatid vesicle (coated vesicle) expanded to be in contact with the Sertoli cytoplasm by a small channel. These tubular projections extended to form a bulbous balloon-like structure forming sometimes a bi-balloon appearance, until torn off from the spermatid into the Sertoli cytoplasm and probably digested by the Sertoli cell. The significance of this structural development was morphologically discussed.
利用灌注固定的睾丸样本，对山羊生精上皮的球管复合体（TBC）的精细结构进行了研究。在精子细胞成熟阶段，TBC在精子释放前出现在头部周边、头部的背侧和腹侧、顶体两侧以及核后鞘区域。在TBC发育的早期阶段，观察到相邻支持细胞的外质特化中滑面内质网部分缺失；细丝束消失，留下小的碎片化滑面内质网。随后，一个精子细胞囊泡（被膜小泡）通过一个小通道扩展并与支持细胞的细胞质接触。这些管状突起延伸形成球状气球样结构，有时形成双球外观，直到从精子细胞上撕下进入支持细胞的细胞质，并可能被支持细胞消化。从形态学角度讨论了这种结构发育的意义。
