DNA与纳米颗粒的偶联。

DNA conjugation to nanoparticles.

作者信息

Park Sunho

机构信息

Dankook University, Yongin-si, Gyeonggi-do, South Korea.

出版信息

Methods Mol Biol. 2013;1025:9-18. doi: 10.1007/978-1-62703-462-3_2.

DOI:10.1007/978-1-62703-462-3_2

PMID:23918326

Abstract

Nanoparticle-DNA (NP-DNA) conjugates have been highlighted due to their versatility in diverse science and engineering fields. The protocol of DNA conjugation to gold nanoparticles (AuNPs), which are among the most popular NPs in bio-applications, is thus described here. This protocol also includes ligand exchange of AuNP to make AuNPs suitable for conjugation process and a fluorescence technique to evaluate the average number of DNA strands attached to single AuNP.

摘要

纳米颗粒 - DNA（NP - DNA）共轭物因其在不同科学和工程领域的多功能性而备受关注。本文描述了将DNA与金纳米颗粒（AuNP）共轭的方法，金纳米颗粒是生物应用中最常用的纳米颗粒之一。该方法还包括金纳米颗粒的配体交换，以使金纳米颗粒适合共轭过程，以及一种荧光技术，用于评估附着在单个金纳米颗粒上的DNA链的平均数量。