Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan.
J Neurosci Res. 2013 Oct;91(10):1303-11. doi: 10.1002/jnr.23266. Epub 2013 Aug 6.
A2BP1 is considered to regulate alternative splicing of important neuronal transcripts and has been implicated in a variety of neurological and developmental disorders. A2BP1 was found in neuronal cells and was analyzed biochemically and morphologically. In this study, we prepared a specific antibody against A2BP1, anti-A2BP1, and carried out protein expression and localization analyses of A2BP1 in rat and mouse tissues. By Western blotting, A2BP1 showed tissue-dependent expression profiles and was expressed in a developmental-stage-dependent manner in the brain. A2BP1 was detected at high levels in neocortex and cerebellum in the rat brain. Immunohistochemical analyses demonstrated that A2BP1 was highly expressed in differentiated neurons but not in mitotically active progenitor cells in the cerebral cortex during developmental stages. In cortical neurons, A2BP1 had accumulated mainly in the nucleus and diffusely distributed in the cell body and dendrites. In differentiated primary cultured rat hippocampal neurons, although A2BP1 was enriched in the nucleus and diffusely distributed in the cytoplasm, it was found in a punctate distribution adjacent to synapses. The results suggest that in neuronal tissues A2BP1 plays important roles, which are regulated in a spatiotemporal manner.
A2BP1 被认为调节重要神经元转录本的可变剪接，并与多种神经发育障碍相关。A2BP1 在神经元细胞中被发现，并在生化和形态上进行了分析。在这项研究中，我们制备了针对 A2BP1 的特异性抗体（抗-A2BP1），并对大鼠和小鼠组织中的 A2BP1 进行了蛋白表达和定位分析。通过 Western blot 分析，A2BP1 显示出组织依赖性的表达谱，并在大脑中表现出发育阶段依赖性的表达。A2BP1 在大鼠脑中的新皮质和小脑中有高表达。免疫组织化学分析表明，A2BP1 在发育阶段的大脑皮质中高度表达于分化的神经元，但不表达于有丝分裂活跃的祖细胞。在皮质神经元中，A2BP1 主要在核内积累，并在胞体和树突中弥散分布。在分化的原代培养大鼠海马神经元中，尽管 A2BP1 在核内富集并在细胞质中弥散分布，但在突触附近呈点状分布。这些结果表明，在神经元组织中，A2BP1 以时空方式发挥重要作用。
