1 National & Regional United Engineering Laboratory of Tissue Engineering, Department of Biomedical Materials Science, College of Biomedical Engineering, Third Military Medical University , Chongqing, China .
Tissue Eng Part A. 2013 Nov;19(21-22):2464-77. doi: 10.1089/ten.tea.2012.0447. Epub 2013 Aug 6.
Properties of the cell-material interface are determining factors in the successful function of cells for cartilage tissue engineering. Currently, cell adhesion is commonly promoted through the use of polypeptides; however, due to their lack of complementary or modulatory domains, polypeptides must be modified to improve their ability to promote adhesion. In this study, we utilized the principle of matrix-based biomimetic modification and a recombinant protein, which spans fragments 7-10 of fibronectin module III (heterophilic motif) and extracellular domains 1-2 of cadherin-11 (rFN/Cad-11) (homophilic motif), to modify the interface of collagen type II (Col II) sponges. We showed that the designed material was able to stimulate cell proliferation and promote better chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs) in vitro than both the FN modified surfaces and the negative control. Further, the Col II/rFN/Cad-11-MSCs composite stimulated cartilage formation in vivo; the chondrogenic effect of Col II alone was much less significant. These results suggested that the rFN/Cad-11-modified collagen type II biomimetic interface has dual biological functions of promoting adhesion and stimulating chondrogenic differentiation. This substance, thus, may serve as an ideal scaffold material for cartilage tissue engineering, enhancing repair of injured cartilage in vivo.
细胞-材料界面的性质是细胞在软骨组织工程中成功发挥功能的决定因素。目前，细胞黏附通常通过使用多肽来促进；然而，由于它们缺乏互补或调节结构域，多肽必须进行修饰以提高其促进黏附的能力。在这项研究中，我们利用基于基质的仿生修饰原理和一种重组蛋白，该蛋白跨越了纤连蛋白模块 III（异源三聚体）的片段 7-10 和钙黏蛋白-11（同源三聚体）的外显子 1-2（rFN/Cad-11），来修饰 II 型胶原（Col II）海绵的界面。我们表明，与 FN 修饰表面和阴性对照相比，设计的材料能够刺激细胞增殖，并促进兔间充质干细胞（MSCs）在体外更好地向软骨分化。此外，Col II/rFN/Cad-11-MSCs 复合物在体内刺激软骨形成；Col II 单独的软骨形成作用要小得多。这些结果表明，rFN/Cad-11 修饰的胶原 II 仿生界面具有促进黏附和刺激软骨分化的双重生物学功能。因此，该物质可能成为软骨组织工程的理想支架材料，增强体内受损软骨的修复。
