CSIR-TWAS fellow, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box-67, Dschang, Cameroon; Department of Molecular Bioprospection, CSIR-Central Institute of Medicinal and Aromatic Plants, Kukrail Picnic Spot Road, P.O. CIMAP, Lucknow 226015, Uttar Pradesh, India.
J Ethnopharmacol. 2013 Oct 7;149(3):833-7. doi: 10.1016/j.jep.2013.07.006. Epub 2013 Aug 3.
Conyza sumatrensis (CS) is an extensively used medicinal herb in the tropics for varied ailments related to pain, inflammation and malaria. Though in constant folkloric use, scientific validations are proving valuable.
Evaluate the safety profile of methanol extract from CS in mice and rats through acute and sub-chronic toxicity studies respectively.
Acute toxicity study involved the single oral administration of CS at 1000, 2000 and 3000mg/kg in mice, while the sub chronic toxicity was carried upon in rats at doses 250, 500 and 1000mg/kg/day for 28 days. Besides body weight, general behaviour and mortality, serum biochemical parameters and liver histology were assessed after 7 and 28 days for acute and sub-chronic study respectively. The parameters were again checked on days 14 and 56 in order to assess the recovery from damage, if any. HPLC fingerprinting of the aqueous and methanol extract was performed through C18 column using water: acetonitrile as mobile phase with observations at 240nm.
In the acute toxicity test, single oral dose of 1000, 2000 and 3000mg/kg of CS did not result in any behavioural changes or mortality, indicating non toxicity. In sub-chronic toxicity studies in rats, no mortality was observed at 250, 500 and 1000mg/kg/day when administered orally for a period of 28 days. Except Serum Glutamate Pyruvate Transaminase (SGPT) level in acute study and Alkaline Phosphatase (ALP), SGPT and Serum Glutamate Oxaloacetate Transaminase (SGOT) level in sub-chronic study, all the observational, haematological and biochemical parameters studied showed non-significant changes. Histological examination of liver did not reveal any treatment-related effects in any of the studies. Moreover, haematological and biochemical changes orchestrated by CS got normalised after 14 and 56 days post-treatment in acute and sub-chronic toxicity respectively. The HPLC fingerprint could resolve 11 and 28 peaks from aqueous and methanol extracts respectively.
The experiments indicate the methanol extract to be safe even at high and repeated doses in pre-clinical studies even though the constituents are more than in aqueous extract.
Sumatera 苍耳（CS）是一种在热带地区广泛使用的药用植物，用于治疗与疼痛、炎症和疟疾有关的各种疾病。尽管在民间长期使用，但科学验证正在证明其价值。
通过急性和亚慢性毒性研究分别评估 CS 甲醇提取物在小鼠和大鼠中的安全性概况。
急性毒性研究包括单次口服 CS，剂量为 1000、2000 和 3000mg/kg 在小鼠中，而亚慢性毒性则在大鼠中进行，剂量为 250、500 和 1000mg/kg/天，为期 28 天。除体重、一般行为和死亡率外，还分别在急性和亚慢性研究的第 7 天和第 28 天评估血清生化参数和肝脏组织学。为了评估是否有任何损伤的恢复，在第 14 天和第 56 天再次检查这些参数。通过 C18 柱使用水：乙腈作为流动相，在 240nm 处进行水提物和甲醇提取物的 HPLC 指纹图谱分析。
在急性毒性试验中，单次口服 1000、2000 和 3000mg/kg 的 CS 不会导致任何行为改变或死亡，表明无毒。在大鼠亚慢性毒性研究中，250、500 和 1000mg/kg/天时，未观察到死亡率当口服给药 28 天。除急性研究中的血清谷氨酸丙酮酸转氨酶（SGPT）水平和亚慢性研究中的碱性磷酸酶（ALP）、SGPT 和血清谷氨酸草酰乙酸转氨酶（SGOT）水平外，所有观察到的、血液学和生化参数均未显示出显著变化。肝脏组织学检查未显示出任何与治疗相关的影响。此外，CS 引起的血液学和生化学变化在急性和亚慢性毒性分别在治疗后 14 天和 56 天恢复正常。HPLC 指纹图谱可以分别从水提物和甲醇提取物中分辨出 11 个和 28 个峰。
实验表明，即使在临床前研究中高剂量和重复剂量下，甲醇提取物也是安全的，尽管其成分比水提物多。
