Dubey Ritesh Kumar, Tripathi D N
Laser & Spectroscopy Laboratory, Department of Physics, Banaras Hindu University, Varanasi- 221 005, India.
Indian J Biochem Biophys. 2005 Oct;42(5):301-7.
The thermal denaturation/renaturation processes in E. coli and eukaryotic DNAs have been studied using the laser light scattering (LS) technique. The differential scattering intensity curve has been utilized to determine the transition temperature T(m). The effect of solution pH on DNA thermal denaturation has been examined. It has been shown clearly that LS is an extremely sensitive method (more than the UV absorption method) and reveals even the subtler effects such as the pre-transition fluctuations, and that the DNA denaturation is prominently affected by pH. The dependence of melting temperature (T(m)) on composition of DNA, number of base pairs and base sequences has also been investigated. It has been observed that depending upon its base sequence, the T(m) decreases in the case of renatured DNAs. The results have been compared with the UV absorption studies.
利用激光散射（LS）技术研究了大肠杆菌和真核生物DNA中的热变性/复性过程。利用差示散射强度曲线确定转变温度T(m)。研究了溶液pH对DNA热变性的影响。结果清楚地表明，激光散射是一种极其灵敏的方法（比紫外吸收法更灵敏），甚至能揭示诸如变性前波动等更细微的效应，而且DNA变性明显受pH影响。还研究了熔解温度（T(m)）对DNA组成、碱基对数和碱基序列的依赖性。观察到，根据其碱基序列，复性DNA的T(m)会降低。已将结果与紫外吸收研究进行了比较。
