Li Mei, Ge Haitao, Kong Xiuqin, Zheng Weifa, Liu Zhili
Department of Biology, College of Life Sciences, Nanjing University, Nanjing, 210093 Jiangsu, China.
Indian J Biochem Biophys. 2005 Aug;42(4):222-7.
The effect of different concentrations of eicosapentaenoic acid (EPA), on rat pheochromocytoma PC12 cells were evaluated using cell viability, lactate dehydrogenase (LDH) activity, flow cytometric DNA analysis and electronic microscopy. A time- and dose-dependent decrease in the cell viability was observed in the cultures of PC12 cells, supplemented with EPA. The incubation with 200 microM EPA for 48 and 72 hr induced a decrease in the cell viability by 53.40 and 53.43%, respectively. Treatment of PC12 cells with EPA induced apoptosis in a concentration-dependent manner, as evidenced by flow cytometry analysis. The highest percentage of apoptotic cells accumulated to 30.32%, following treatment with 200 microM EPA. The LDH levels increased significantly on treatment with 100 and 200 microM EPA, by 144.4 and 197.3%, respectively, compared with the untreated control. In addition, the cell morphology change was also observed by electron microscopy. The results suggest that EPA mediates its effect on the PC12 cells, at least in part, via the induction by apoptosis.
使用细胞活力、乳酸脱氢酶（LDH）活性、流式细胞术DNA分析和电子显微镜，评估了不同浓度的二十碳五烯酸（EPA）对大鼠嗜铬细胞瘤PC12细胞的影响。在补充了EPA的PC12细胞培养物中，观察到细胞活力呈时间和剂量依赖性下降。用200微摩尔/升EPA孵育48小时和72小时，分别导致细胞活力下降53.40%和53.43%。流式细胞术分析表明，用EPA处理PC12细胞以浓度依赖性方式诱导细胞凋亡。用200微摩尔/升EPA处理后，凋亡细胞的最高百分比累积至30.32%。与未处理的对照相比，用100和200微摩尔/升EPA处理后，LDH水平分别显著增加144.4%和197.3%。此外，通过电子显微镜也观察到了细胞形态变化。结果表明，EPA至少部分地通过诱导细胞凋亡介导其对PC12细胞的作用。
