Shanju S, Geraldine P
Department of Animal Science, Bharathidasan University, Tiruchirapalli -620024.
Indian J Biochem Biophys. 2005 Aug;42(4):258-61.
Ovarian yolk protein (vitellin) of adult freshwater prawns Macrobrachium malcolmsonii, M. rosenbergii and M. lamarreii was characterized, and relationship between the gonadosomatic index (GSI) and the appearance of specific polypeptides in their ovary during different stages (early-mature, mature and spent) of reproductive cycle was studied. Mature ovaries of all the three species showed increased GSI values, compared to the respective early-mature ovaries. Protein profiles at the three stages of ovarian maturation were analysed by SDS-PAGE. Polypeptides of low molecular mass (20 to 70 kDa) were prominent in the early-mature ovary, whereas in mature ovary, 89 and 100 kDa polypeptides were predominant. Immunodiffusion studies, using antiserum raised against purified vitellin from mature ovary of M. malcolmsonii indicated antigenic similarities of vitellins among the three species.
对成年淡水虾马氏沼虾、罗氏沼虾和拉氏沼虾的卵巢卵黄蛋白（卵黄磷蛋白）进行了表征，并研究了生殖周期不同阶段（早熟、成熟和产卵后）性腺指数（GSI）与卵巢中特定多肽出现之间的关系。与各自的早熟卵巢相比，所有这三个物种的成熟卵巢显示出更高的GSI值。通过SDS-PAGE分析了卵巢成熟三个阶段的蛋白质谱。早熟卵巢中低分子量（20至70 kDa）的多肽很突出，而在成熟卵巢中，89 kDa和100 kDa的多肽占主导地位。使用针对从马氏沼虾成熟卵巢中纯化的卵黄磷蛋白产生的抗血清进行的免疫扩散研究表明，这三个物种的卵黄磷蛋白具有抗原相似性。
