Bhatia Shikha, Lodha M L
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India.
Indian J Biochem Biophys. 2005 Jun;42(3):152-5.
Antiviral proteins (AVPs) purified from the leaves of Bougainvillea xbuttiana cv Mahara exhibited RNase activity against viral RNA of the tobamoviruses, Tobacco mosaic virus (TMV) and Sunnhemp rosette virus (SRV). They caused complete degradation of viral RNAs in a concentration-dependent manner. RNase activity gel assay ruled out the possibility of the presence of contaminating nucleases. AVPs also showed DNase activity, as indicated by conversion of supercoiled form of plasmid DNA into relaxed and linear forms. The implications of these activities in controlling plant viruses are discussed.
从光叶三角梅品种Mahara叶片中纯化得到的抗病毒蛋白（AVP）对烟草花叶病毒属病毒烟草花叶病毒（TMV）和黄花稔莲座病毒（SRV）的病毒RNA表现出核糖核酸酶活性。它们以浓度依赖的方式导致病毒RNA完全降解。核糖核酸酶活性凝胶分析排除了存在污染性核酸酶的可能性。AVP还表现出脱氧核糖核酸酶活性，如质粒DNA的超螺旋形式转化为松弛和线性形式所示。讨论了这些活性在控制植物病毒方面的意义。
