Dutt Som, Yadav O P, Kapoor H C, Lodha M L
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India.
Indian J Biochem Biophys. 2004 Feb;41(1):29-33.
Antiviral proteins (AVPs) named CAP-I and CAP-II purified from the leaves of Chenopodium album cv Pusa Bathua-1 induced systemic resistance against tobacco mosaic virus (TMV) and sunnhemp rosette virus (SRV) in both hypersensitive as well as systemic hosts. An increased accumulation of two polypeptides (approximately 17 kDa and approximately 26 kDa) was observed in untreated upper leaves of Cyamopsis tetragonoloba plants whose basal leaves were treated with CAP-I/CAP-II. Both AVPs exhibited ribosomal RNA N-glycosidase activity on 28S rRNA of tobacco leaves and also caused in vitro degradation of TMV RNA. It is suggested that the CAP-I and -II are multi-functional and may be acting at multiple levels to ensure maximum possible inhibition of viral infection.
从藜（品种为Pusa Bathua-1）叶片中纯化得到的抗病毒蛋白（AVP）CAP-I和CAP-II，在过敏宿主和系统宿主中均能诱导对烟草花叶病毒（TMV）和太阳麻莲座病毒（SRV）的系统抗性。在基部叶片用CAP-I/CAP-II处理过的四角豆植株未处理的上部叶片中，观察到两种多肽（约17 kDa和约26 kDa）积累增加。两种AVP均对烟草叶片的28S rRNA表现出核糖体RNA N-糖苷酶活性，并且还导致TMV RNA的体外降解。研究表明，CAP-I和CAP-II具有多种功能，可能在多个层面发挥作用，以确保对病毒感染的最大程度抑制。
