Ge Shaolin, Cheng Xinsheng, Xue Zechun, Yang Liwen, Zheng Xiaoyun
Research Center of Tobacco and Health, University of Science & Technology, Hefei 230052, People's Republic of China.
Indian J Biochem Biophys. 2005 Jun;42(3):166-72.
Systemic acquired resistance (SAR) is an important component of disease-resistance arsenal of plants, and is associated with enhanced potency of activating local and systemic defense-related responses upon pathogen attack. In this report, we demonstrated that pre-treatment with beta-aminobutyric acid (BABA), a new elicitor of SAR in the plants, enhanced resistance against tobacco mosaic virus (TMV) in a temperately-sensitive tobacco (Nicotiana tabacum L.) cultivar Yunyan 85. The resistance is based on the elicitation of defense-related responses induced by BABA that brings the TMV-susceptible tobacco plants to a defense-ready state, even before exposure to the pathogen. The induced resistance was strongly associated with potentiated activation of defense-related enzymes [phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO)] activities, proportional to the concentration of the BABA sprayed. Interestingly, simultaneous clipping, an important agricultural practice in tobacco production, attenuated BABA-mediated enhancement of TMV resistance in tobacco. The changes in the defense-related enzymes activities indicated that the interaction between BABA and wounding was reciprocally antagonistic. Moreover, such a negative interaction regulated the expression of defense-related enzymes. depending on the time of induction.
系统获得性抗性（SAR）是植物抗病武器库的重要组成部分，与病原体攻击后激活局部和系统防御相关反应的能力增强有关。在本报告中，我们证明了用β-氨基丁酸（BABA）预处理，一种植物中SAR的新激发子，可增强对烟草花叶病毒（TMV）的抗性，该病毒对温度敏感的烟草（Nicotiana tabacum L.）品种云烟85具有抗性。这种抗性基于BABA诱导的防御相关反应，即使在未接触病原体之前，也能使易感TMV的烟草植株进入防御就绪状态。诱导抗性与防御相关酶[苯丙氨酸解氨酶（PAL）和多酚氧化酶（PPO）]活性的增强密切相关，与喷施的BABA浓度成正比。有趣的是，同时打顶，烟草生产中的一项重要农艺措施，减弱了BABA介导的烟草对TMV抗性的增强。防御相关酶活性的变化表明BABA与创伤之间的相互作用是相互拮抗的。此外，这种负相互作用调节了防御相关酶的表达，这取决于诱导时间。
