Gao Z Q, Wang G C, Tseng C K
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, The Chinese Academy of Sciences, Qingdao 266071, China.
Indian J Biochem Biophys. 2005 Feb;42(1):41-7.
An improved method for isolation and characterization of photosystem (PS)II particles from thylakoid membranes of gametophytes of a marine alga Porphyra yezoensis Udea is reported. Thylakoid membranes were isolated using ultracentrifugation and differential speeds centrifugation and were further purified by the first sucrose density gradient centrifugation (SDGC). PSII particles with high 2, 6-dichloroindophenol (DCIP) photo-reduction activity were isolated by the second SDGC from the thylakoid membranes. Absorption and fluorescence spectra of the thylakoid membranes and PSII particles were recorded and their polypeptides composition was studied. Thylakoid membranes obtained by the above two methods showed similar spectral properties and polypeptides composition. PSII particles, in addition to common extrinsic proteins found in PSII of other plants, contained cyt c-550, a 20 kDa protein, along with two new proteins (14 kDa and 16 kDa).
报道了一种从海洋藻类条斑紫菜叶状体类囊体膜中分离和鉴定光系统（PS）II颗粒的改进方法。使用超速离心和差速离心分离类囊体膜，并通过首次蔗糖密度梯度离心（SDGC）进一步纯化。通过第二次SDGC从类囊体膜中分离出具有高2,6 - 二氯靛酚（DCIP）光还原活性的PSII颗粒。记录了类囊体膜和PSII颗粒的吸收光谱和荧光光谱，并研究了它们的多肽组成。通过上述两种方法获得的类囊体膜表现出相似的光谱特性和多肽组成。PSII颗粒除了含有其他植物PSII中常见的外在蛋白外，还含有细胞色素c - 550、一种20 kDa的蛋白以及两种新蛋白（14 kDa和16 kDa）。
