Gamboa Graciela S, Ambrosio Ana María, Maiza Andrea S, Mariani Mauricio, Rodrigues Garcia Armôa Geraldo, Saavedra María Del Carmen
Instituto Nacional de Enfermedades Virales Humanas Dr. Julio I. Maiztegui, Argentina.
Medicina (B Aires). 2013;73(4):303-9.
Argentine hemorrhagic fever is a severe acute disease caused by Junin virus. For prevention of this disease an effective vaccine called Candid#1 has been developed, composed of a live attenuated Junin virus strain. During a clinical trial conducted at Instituto Nacional de Enfermedades Virales Humanas (INEVH) in 2005, Junin virus was isolated from two vaccinated volunteers by co-culture of peripheral mononuclear blood cells. The aim of this study was to compare the strains isolated from these human volunteers with Candid#1 strain regarding phenotypic characteristics of attenuation according to the indicators developed by Contigiani and Sabattini in 1977. The three strains were lethal to suckling mice but not to 10-12 days old mice and guinea pigs. Surviving guinea pigs from primary infection were protected when challenged by intra-muscular inoculation with lethal doses of a virulent strain. Infection and protection rates indicate that these strains are highly infective and protective in the hosts studied herein. These results demonstrate that Junin virus strains isolated from volunteers immunized with Candid#1 maintain the same attenuated phenotype of Candid#1 vaccine after one passage in humans.
阿根廷出血热是由胡宁病毒引起的一种严重急性疾病。为预防这种疾病，已研发出一种名为Candid#1的有效疫苗，它由一种减毒活胡宁病毒株组成。在2005年国立人类病毒病研究所（INEVH）进行的一项临床试验中，通过外周血单个核细胞共培养，从两名接种疫苗的志愿者体内分离出了胡宁病毒。本研究的目的是根据Contigiani和Sabattini在1977年制定的指标，比较从这些人类志愿者分离出的毒株与Candid#1毒株在减毒表型特征方面的差异。这三种毒株对乳鼠具有致死性，但对10 - 12日龄小鼠和豚鼠无致死性。初次感染后存活的豚鼠在经肌肉接种致死剂量的强毒株攻击时受到保护。感染率和保护率表明，这些毒株在本文所研究的宿主中具有高度传染性和保护性。这些结果表明，从接种Candid#1疫苗的志愿者体内分离出的胡宁病毒株在人体内传代一次后，仍保持与Candid#1疫苗相同的减毒表型。
