青鳉SENP1基因催化结构域的分子克隆与细菌表达
Molecular cloning and bacterial expression of the catalytic domain of the SENP1 gene of Oryzias latipes.
作者信息
Obata Shinpei, Yuasa Eri, Seki Daisuke, Kitano Takeshi, Saitoh Hisato
机构信息
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University.
出版信息
Biosci Biotechnol Biochem. 2013;77(8):1788-91. doi: 10.1271/bbb.130357. Epub 2013 Aug 7.
In this study, we cloned the catalytic domain of the Oryzias latipes sentrin/SUMO-specific protease 1 (OlSENP1-CD) gene and produced the recombinant OlSENP1-CD protein in Escherichia coli. Experimental procedures designed to reveal the ability of the recombinant protein to show deSUMOylating activity in vitro should be helpful in future studies of other SENPs and the SUMO pathway.
在本研究中，我们克隆了青鳉泛素样修饰特异性蛋白酶1（OlSENP1）基因的催化结构域（OlSENP1-CD），并在大肠杆菌中表达了重组OlSENP1-CD蛋白。旨在揭示重组蛋白在体外显示去泛素样修饰活性能力的实验程序，将有助于未来对其他泛素样修饰特异性蛋白酶（SENPs）和泛素样修饰（SUMO）途径的研究。
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