Das Puranjan, Pramanick Kousik, Mukherjee Dilip, Maiti B R
Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Calcutta, 700019, India.
Fish Physiol Biochem. 2014 Feb;40(1):105-16. doi: 10.1007/s10695-013-9828-0. Epub 2013 Aug 8.
In this article, an in vitro investigation was carried out to ascertain the roles of hormones and growth factor in the inductions of oocyte maturation and steroidogenesis of the postvitellogenic follicles in an Indian estuarine grey mullet, Mugil cephalus L. Oocyte maturation was evaluated by scoring the germinal vesicle breakdown (GVBD) percent of the postvitellogenic follicles. All the sex [17α,20β-dihydroxy-4-pregnane-3-one (DHP), estradiol 17β (E₂), progesterone (P), 17α-OH progesterone (17-OH-P) and testosterone] and other [bovine-insulin and salmon-calcitonin, human chorionic gonadotropin (hCG), luteinizing hormone (LH) or hCG+DHP] hormones and insulin-like growth factor-I (IGF-I) significantly increased GVBD% in 9 h culture. DHP had a maximum effect (75 %) compared to other effectors. Some effectors (hCG: 82.14 %, LH: 78.94 %, hCG plus DHP: 81.81 %, E₂: 80 % and IGF-I: 74.19 %) including DHP (79 %) further increased GVBD% in 15-h culture. All the hormones (except DHP) and IGF-I increased DHP, E₂ and testosterone productions by the postvitellogenic ovarian follicles in vitro. DHP and testosterone productions were increased with the increase of incubation time from 9 h through 15 h. E₂ production was not further increased beyond 12 h. DHP production was highest by hCG compared to other effectors. The hCG of all the test compounds was most effective in both the induction of GVBD% and steroid production. DHP is the most potent inducer of oocyte maturation in Indian estuarine flat head grey mullet. Involvement of estrogen in mullet oocyte maturation is indicated. hCG, like DHP, is equally potent and induces oocyte maturation via DHP production in vitro. hCG with DHP has synergistic action on oocyte maturation in mullet ovary. Interplay of several hormones (hCG, LH, and probably E₂ and testosterone) and IGF-I on oocyte maturation is suggested in the mullet.
在本文中，开展了一项体外研究，以确定激素和生长因子在印度河口灰鲻（ Mugil cephalus L.）卵黄生成后卵泡的卵母细胞成熟诱导和类固醇生成中的作用。通过对卵黄生成后卵泡的生发泡破裂（GVBD）百分比进行评分来评估卵母细胞成熟情况。所有性类固醇激素[17α,20β - 二羟基 - 4 - 孕烷 - 3 - 酮（DHP）、雌二醇17β（E₂）、孕酮（P）、17α - 羟基孕酮（17 - OH - P）和睾酮]以及其他激素[牛胰岛素和鲑鱼降钙素、人绒毛膜促性腺激素（hCG）、促黄体生成素（LH）或hCG + DHP]和胰岛素样生长因子 - I（IGF - I）在9小时培养中均显著提高了GVBD百分比。与其他效应物相比，DHP具有最大效应（75%）。一些效应物（hCG：82.14%、LH：78.94%、hCG加DHP：81.81%、E₂：80%和IGF - I：74.19%）包括DHP（79%）在15小时培养中进一步提高了GVBD百分比。所有激素（除DHP外）和IGF - I均在体外增加了卵黄生成后卵巢卵泡的DHP、E₂和睾酮生成。DHP和睾酮生成随着孵育时间从9小时增加到15小时而增加。E₂生成在12小时后没有进一步增加。与其他效应物相比，hCG使DHP生成最高。所有测试化合物中，hCG在诱导GVBD百分比和类固醇生成方面最为有效。DHP是印度河口平头灰鲻中最有效的卵母细胞成熟诱导剂。表明雌激素参与了灰鲻卵母细胞成熟过程。hCG与DHP一样有效，并且在体外通过DHP生成诱导卵母细胞成熟。hCG与DHP对灰鲻卵巢中的卵母细胞成熟具有协同作用。推测在灰鲻中几种激素（hCG、LH，可能还有E₂和睾酮）与IGF - I在卵母细胞成熟过程中存在相互作用。
