Toktam Memariani, Kioomars Salimi Nejad, Kourosh Kamali, Adel Shervin, Behrokh Mohajer-Maghari, Mohhamad Mehdi Akhondi, Hamid Reza Khorram Khorshid
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
J Reprod Infertil. 2010 Apr;11(1):33-7.
Angiogenesis, growth of new blood vessels from pre-existing vessels, is a crucial physiological process for tissue regeneration. This state is also seen in pathological processes such as malignancies and endometriosis. Vascular endothelial growth factor (VEGF) is a major mediator of angiogenesis and vascular permeability which is known to play an important role in the development of endometriosis. The aim of this study was to investigate the relationship between +405 G>C VEGF polymorphism and endometriosis in an Iranian population.
The study population was comprised of 105 women with and 150 women without laparoscopic evidence of endometriosis. Genomic DNA from blood cells was extracted using salting out method. Genotype and allele frequency of +405 G>C polymorphism was compared between women with endometriosis and the controls using PCR-RFLP. Statistical analysis was performed using SPSS 13.0 software. Chi-squared test and odds ratio plus 95% confidence interval were determined. A p-value less than 0.05 was considered statistically significant.
While the +405 VEGF genotype frequencies in the case group were 41.3% G/G, 46.2% C/G and %12.5 C/C, they were 32% GG, %53.3 GC and 14.7% CC in the control group. The distribution of three genotypes and allele frequencies of +405 G>C VEGF polymorphism between the case and control groups did not demonstrate any significant difference.
In contrast to previous studies, no significant correlation was found between +405 G>C VEGF polymorphism and endometriosis. Since this was the first study in an Iranian population, further investigation with bigger sample sizes may be indicated to be able to generalize the findings.
血管生成，即从已有的血管中生长出新的血管，是组织再生的关键生理过程。这种状态在诸如恶性肿瘤和子宫内膜异位症等病理过程中也会出现。血管内皮生长因子（VEGF）是血管生成和血管通透性的主要调节因子，已知其在子宫内膜异位症的发展中起重要作用。本研究的目的是调查伊朗人群中VEGF基因+405 G>C多态性与子宫内膜异位症之间的关系。
研究人群包括105例经腹腔镜检查证实患有子宫内膜异位症的女性和150例无该病证据的女性。采用盐析法从血细胞中提取基因组DNA。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法比较子宫内膜异位症患者和对照组中+405 G>C多态性的基因型和等位基因频率。使用SPSS 13.0软件进行统计分析。确定卡方检验、比值比及95%置信区间。p值小于0.05被认为具有统计学意义。
病例组中+405 VEGF基因型频率分别为41.3% G/G、46.2% C/G和12.5% C/C，对照组中分别为32% GG、53.3% GC和14.7% CC。病例组和对照组之间+405 G>C VEGF多态性的三种基因型分布和等位基因频率没有显示出任何显著差异。
与之前的研究相反，未发现+405 G>C VEGF多态性与子宫内膜异位症之间存在显著相关性。由于这是对伊朗人群的首次研究，可能需要更大样本量的进一步调查才能推广这些发现。
