Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina.
J Med Entomol. 2013 Jul;50(4):916-9. doi: 10.1603/me12249.
ABSTRACT This initial study is aimed to measure the performance of incorporating pyriproxyfen in natural materials with low environmental impact to obtain slow release formulations that can be used as larvicidal or autocidal ovitraps avoiding hatched Aedes aegypti (L.) eggs to emerge as adults. Hollow candles made of beeswax or paraffin:stearin 1:1 mixture containing pyriproxyfen 0.01 and 0.05% were prepared and used as holding water containers for larval bioassay. Pyriproxyfen was released quickly into the larvae-breeding water. Ae. aegypti larvae were introduced immediately after the addition of tap water to the hollow candles (t = 1 min) or after 1, 4, and 8 h. More than 40% of the larvae did not emerge as adults for t = 1 min, reaching 80-100% when the larvae were added after 1 or 4 h, respectively. The hollow candles were kept at room temperature, and water was replaced every 15 d. Bioassays performed every 30 d showed that the residual activity obtained for both matrices and both concentrations of pyriproxyfen was higher than 360 d, with 100% inhibition of adult emergence.
摘要 本初步研究旨在测量将吡丙醚掺入低环境影响的天然材料中的性能，以获得可作为杀幼虫或自杀虫诱卵器的缓释制剂，从而避免已孵化的埃及伊蚊（L.）卵发育成成虫。制备了含有 0.01%和 0.05%吡丙醚的蜂蜡或石蜡-硬脂酸 1:1 混合物的空心蜡烛，并将其用作幼虫生物测定的持水容器。吡丙醚迅速释放到幼虫养殖水中。在向空心蜡烛中加入自来水后立即（t = 1 分钟）或 1、4 和 8 小时后加入埃及伊蚊幼虫。对于 t = 1 分钟，超过 40%的幼虫未能发育成成虫，而当幼虫在 1 或 4 小时后加入时，分别达到 80-100%。空心蜡烛保持在室温下，每 15 天更换一次水。每隔 30 天进行的生物测定表明，两种基质和两种浓度的吡丙醚的残留活性均高于 360 天，成虫出现的抑制率达到 100%。
