Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-Dong), Mapo-Gu, Seoul 121-742, Korea.
J Biomed Nanotechnol. 2013 Aug;9(8):1398-402. doi: 10.1166/jbn.2013.1519.
A cell chip is a valuable tool to evaluate the effects of anticancer drugs, antibiotics and environmental toxicants on various kinds of cells. In this study, a conductive composite material composed of multi-walled carbon nanotubes (CNTs) and RGD-MAP-C peptide was fabricated on gold electrode surface for enhancing electrochemical signals from HEK293T cells. The topological characteristics and electrochemical performance of composite materials with different concentrations of CNTs were analyzed by scanning electron microscopy and cyclic voltammetry (CV), respectively. CNTs/RGD peptide composites (CP) electrode containing 20 microg/ml CNT was found to be excellent for improving the sensitivity of cell chip compared with that of bare gold or RGD peptide modified electrode. Finally, two kinds of nephrotoxic antibiotics were treated to HEK293T cells and their toxicity were successfully monitored by CV. Our CP composites can be used as a suitable conducting material for the fabrication of various kinds of cell-based chips.
细胞芯片是一种评估抗癌药物、抗生素和环境毒物对各种细胞影响的有效工具。在本研究中，我们在金电极表面制备了一种由多壁碳纳米管（CNTs）和 RGD-MAP-C 肽组成的导电复合材料，用于增强 HEK293T 细胞的电化学信号。通过扫描电子显微镜和循环伏安法（CV）分别分析了不同 CNTs 浓度的复合材料的拓扑特征和电化学性能。结果表明，与裸金电极或 RGD 肽修饰电极相比，含有 20μg/ml CNT 的 CNT/RGD 肽复合材料（CP）电极更有利于提高细胞芯片的灵敏度。最后，我们将两种肾毒性抗生素处理 HEK293T 细胞，并通过 CV 成功监测了它们的毒性。我们的 CP 复合材料可用作制造各种基于细胞的芯片的合适导电材料。
