Barcelona Centre for International Health Research (CRESIB Hospital Clínic-Universitat de Barcelona), Barcelona, Spain.
Clin Microbiol Infect. 2014 Feb;20(2):O135-8. doi: 10.1111/1469-0691.12327. Epub 2013 Aug 8.
Persistence of giardiasis after some of the recommended drugs is occurring with increasing frequency. We describe the follow-up of four members of a family with giardiasis through microscopic observation, immunochromatography and PCRs of tpi and β-giardin genes. Three patients did not respond to tinidazole but they were cured after quinacrine. However, PCR became negative at 2 months after negativization of stools in two patients and after 1 year in one patient. In all cases Giardia assemblage B was characterized with high homology between all isolates. Further studies are needed to determine the value of PCR in the diagnosis of Giardia infections.
贾第虫病在经过一些推荐药物治疗后仍然存在，且其发病率正在不断上升。我们通过显微镜观察、免疫层析法和 tpi 及β-贾第虫基因的 PCR 对一个贾第虫病患者家庭的 4 名成员进行了随访。3 名患者对替硝唑没有反应，但用奎宁后痊愈。然而，在 2 名患者粪便转阴后 2 个月和 1 名患者 1 年后，PCR 转为阴性。所有情况下，贾第虫属 B 组的所有分离株之间都具有高度同源性。需要进一步研究来确定 PCR 在贾第虫感染诊断中的价值。
