ExoSensor 517：一种用于可视化神经递质胞吐的双分析物荧光化学传感器。
ExoSensor 517: a dual-analyte fluorescent chemosensor for visualizing neurotransmitter exocytosis.
机构信息
Department of Chemistry, University of Missouri-Columbia , 601 South College Avenue, Columbia, Missouri 65211, United States.
出版信息
ACS Chem Neurosci. 2013 Oct 16;4(10):1334-8. doi: 10.1021/cn400128s. Epub 2013 Aug 14.
Abstract
A dual-analyte fluorescent chemosensor (ExoSensor 517) for the direct visualization of neurotransmitters released upon exocytosis is presented. The sensor exploits the high concentration of neurotransmitters (e.g., glutamate, norepinephrine, and dopamine) and the pH gradient between the vesicle and synaptic cleft. The cooperative recognition elements require both binding and a change in environmental pH to afford a fluorescence response which makes ExoSensor 517 one of the first integrated molecular logic gates to be used for biological applications.
摘要
本文介绍了一种用于直接可视化胞吐作用释放的神经递质的双分析物荧光化学传感器（ExoSensor 517）。该传感器利用神经递质（例如谷氨酸、去甲肾上腺素和多巴胺）的高浓度和囊泡与突触间隙之间的 pH 梯度。协同识别元件需要结合和环境 pH 的变化才能提供荧光响应，这使得 ExoSensor 517 成为第一个用于生物应用的集成分子逻辑门之一。
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