Hu Dong-mei, Cai Jian-piao, Wang Da-hu, DI Biao, Qiu Li-wen, Wang Ya-di, Chen Yue, Ding Xi-xia, Che Xiao-yan
Center for Clinical Medicine Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2013 Apr;47(4):363-6.
To establish a highly sensitive and specific assay to detect dengue virus (DENV) envelope protein domain III (EDIII) IgG antibody, and to explore its value in the diagnosis and seroepidemiological survey of dengue.
The DENV EDIII IgG antibody capture ELISA was developed using the recombinant full-length DENV EDIII, which was prepared by Pichia yeast expression system as the capture antigen. The serum samples were collected from the same group of 35 DENV-1 patients of primary infection during disease period in 2006 and their follow-up phase in 2010; and the sensitivity of the assay was compared to that of the commercial Panbio DENV IgG ELISA.
The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from disease period and follow-up phase was 87% (20/23) and 94% (33/35), respectively; whereas the sensitivity of Panbio DENV IgG ELISA was 71% (25/35) and 0, respectively. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from both periods was similar, without statistical significance (χ(2) = 0.946, P = 0.331). For serum samples from disease period, the sensitivity of DENV EDIII IgG ELISA was comparable with that of Panbio DENV IgG ELISA (χ(2) = 1.924, P = 0.165). However, DENV EDIII IgG ELISA demonstrated a significantly higher sensitivity than Panbio DENV IgG ELISA in detecting the serum samples from follow-up phase (χ(2) = 62.432, P = 0.000).
DENV EDIII IgG capture ELISA is highly sensitive in detecting IgG in the serum samples from either disease period or follow-up phase. This method might be a promising alternative for diagnosis and seroepidemiologic survey of dengue.
建立一种高灵敏度和特异性的检测登革病毒（DENV）包膜蛋白结构域III（EDIII）IgG抗体的方法，并探讨其在登革热诊断和血清流行病学调查中的价值。
采用毕赤酵母表达系统制备的重组全长DENV EDIII作为捕获抗原，建立DENV EDIII IgG抗体捕获ELISA法。收集2006年同一组35例初次感染DENV-1患者发病期及2010年随访期的血清样本，并将该检测方法的灵敏度与商业化的泛博DENV IgG ELISA法进行比较。
DENV EDIII IgG ELISA法检测发病期和随访期血清样本的灵敏度分别为87%（20/23）和94%（33/35）；而泛博DENV IgG ELISA法的灵敏度分别为71%（25/35）和0。DENV EDIII IgG ELISA法检测两个时期血清样本的灵敏度相似，无统计学意义（χ(2)=0.946，P=0.331）。对于发病期血清样本，DENV EDIII IgG ELISA法的灵敏度与泛博DENV IgG ELISA法相当（χ(2)=1.924，P=0.165）。然而，DENV EDIII IgG ELISA法在检测随访期血清样本时，其灵敏度显著高于泛博DENV IgG ELISA法（χ(2)=62.432，P=0.000）。
DENV EDIII IgG捕获ELISA法在检测发病期或随访期血清样本中的IgG时具有高灵敏度。该方法可能是登革热诊断和血清流行病学调查的一种有前景的替代方法。
