State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
Analyst. 2013 Oct 21;138(20):5933-41. doi: 10.1039/c3an00957b. Epub 2013 Aug 9.
Ionic liquids (ILs) have numerous chemical applications as environmentally green solvents that are extending into microemulsion applications. In this work, a novel benign IL-in-water microemulsion system modified by an IL surfactant has been proposed for simultaneous extraction of hydrophilic and lipophilic constituents from Flos Chrysanthemi (Chrysanthemum morifolium). Constituents were analyzed by rapid-resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A mixture-design approach was used to optimize the IL surfactant and the IL oil phase in the microemulsion system. Microemulsions consisting of 6.0% 1-dodecyl-3-methylimidazolium hydrogen sulfate, 0.1% 1-vinyl-3-methylimidazolium hexafluorophosphate and 93.9% water offered the acceptable extract efficiency that are comparable to or even better than conventional volatile organic solvents. This assay was fully validated with respect to the linearity of response (r(2) > 0.999 over two orders of magnitude), precision (intra-RSD < 0.49 and inter-day RSD < 2.21), and accuracy (recoveries ranging from 93.73% to 101.84%). The proposed IL-in-water microemulsion method provided an environmentally friendly alternative for efficient extraction of compounds from Flos Chrysanthemi and could be extended to complex environmental and pharmaceutical samples.
离子液体（ILs）作为环境友好的绿色溶剂在化学领域有广泛的应用，并且正在向微乳液应用扩展。在这项工作中，提出了一种新型的由 IL 表面活性剂改性的良性 IL-水微乳液体系，用于从菊花（Chrysanthemum morifolium）中同时提取亲水性和疏水性成分。通过快速分辨液相色谱-四极杆飞行时间质谱联用对成分进行分析。采用混合设计方法优化微乳液体系中的 IL 表面活性剂和 IL 油相。由 6.0% 1-十二烷基-3-甲基咪唑硫酸氢盐、0.1% 1-乙烯基-3-甲基咪唑六氟磷酸盐和 93.9%水组成的微乳液提供了可接受的萃取效率，与传统挥发性有机溶剂相当，甚至更好。该方法在响应线性（两个数量级范围内 r(2) > 0.999）、精密度（日内 RSD < 0.49，日间 RSD < 2.21）和准确度（回收率在 93.73%至 101.84%之间）方面进行了全面验证。所提出的 IL-水微乳液方法为从菊花中有效提取化合物提供了一种环境友好的替代方法，并可扩展到复杂的环境和药物样品中。
