Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, TX, 77204, USA.
Eur J Appl Physiol. 2013 Oct;113(10):2629-35. doi: 10.1007/s00421-013-2706-8. Epub 2013 Aug 9.
This study compared the acute immune response, inflammation, and lipid peroxidation to a 75 km cycling time trial in male athletes testing positive or negative for latent cytomegalovirus (CMV) infection.
Trained cyclists (N = 20) were tested for CMV serostatus, and cycled 75 km on a mountainous course using indoor trainers with continuous workload monitoring. Pre-, post-, and 1 h post-exercise blood samples were analyzed for total blood leukocyte counts, blood granulocyte (GR) and monocyte (MO) phagocytosis (PHAG) and oxidative burst activity (OBA), four plasma cytokines, and plasma F2-isoprostanes.
Forty percent of the subjects tested positive for CMV. No differences in subject characteristics were found between CMVpos and CMVneg groups. Mean power (57.3 ± 1.6, 59.4 ± 1.8 % maximal Watts, p = 0.803), heart rate (87.0 ± 1.0, 86.5 ± 1.3 % maximal heart rate, p = 0.376), and total time (2.56 ± 0.08, 2.60 ± 0.08 h, p = 0.744) to complete the 75 km cycling time trial did not differ between CMVpos and CMVneg groups. Whereas exercise induced significant changes in total blood leukocyte counts, GR and MO-PHAG, four plasma cytokines, and plasma F2-isoprostanes (p < 0.05, ω(2) > 0.03), these exercise-induced changes did not differ between CMVpos and CMVneg groups (p > 0.05, ω(2) < 0.01).
CMV serostatus does not appear to influence these innate immune responses or markers of inflammation and lipid peroxidation in response to a single bout of heavy exertion.
本研究比较了潜伏巨细胞病毒（CMV）感染阳性或阴性的男性运动员在进行 75 公里自行车计时赛时的急性免疫反应、炎症和脂质过氧化。
对 20 名受过训练的自行车运动员进行 CMV 血清学检测，并在使用室内训练器进行的山地课程上以 75 公里的速度骑行，同时连续监测工作量。在运动前、运动后和运动后 1 小时采集血液样本，分析总白细胞计数、血液粒细胞（GR）和单核细胞（MO）吞噬作用（PHAG）和氧化爆发活性（OBA）、四种血浆细胞因子和血浆 F2-异前列腺素。
40%的受试者 CMV 检测呈阳性。CMVpos 和 CMVneg 组之间在受试者特征方面没有差异。平均功率（57.3±1.6、59.4±1.8%最大瓦特，p=0.803）、心率（87.0±1.0、86.5±1.3%最大心率，p=0.376）和完成 75 公里自行车计时赛的总时间（2.56±0.08、2.60±0.08 小时，p=0.744）在 CMVpos 和 CMVneg 组之间没有差异。虽然运动引起了总白细胞计数、GR 和 MO-PHAG、四种血浆细胞因子和血浆 F2-异前列腺素的显著变化（p<0.05，ω(2)>0.03），但这些运动引起的变化在 CMVpos 和 CMVneg 组之间没有差异（p>0.05，ω(2)<0.01）。
CMV 血清学状态似乎不会影响单次剧烈运动后这些先天免疫反应或炎症和脂质过氧化的标志物。
